Exercise training normalizes elevated firing rate of hypothalamic presympathetic neurons in heart failure rats

Exercise training normalizes elevated firing rate of hypothalamic presympathetic neurons in heart failure rats. Am J Physiol Regul Integr Comp Physiol 316: R110 –R120, 2019. First published November 28, 2018; doi: 10.1152/ajpregu.00225.2018.—Exercise training (ExT) normalizes elevated sympathetic nerve activity in heart failure (HF), but the underlying mechanisms are not well understood. In this study, we examined the effects of 3 wk of ExT on the electrical activity of the hypothalamic presympathetic neurons in the brain slice of HF rats. HF rats were prepared by ligating the left descending coronary artery. The electrophysiological properties of paraventricular nucleus neurons projecting to the rostral ventrolateral medulla (PVN-RVLM) were examined using the slice patch-clamp technique. The neuronal firing rate was elevated in HF rats, and ExT induced a reduction in the firing rate (P < 0.01). This ExT-induced decrease in the firing rate was associated with an increased frequency of spontaneous and miniature inhibitory postsynaptic current (IPSCs; P < 0.05). There was no significant change in excitatory postsynaptic current. Replacing Ca ²⁺ with Mg ²⁺ in the recording solution reduced the elevated IPSC frequency in HF rats with ExT (P < 0.01) but not in those without ExT, indicating an increase in the probability of GABA release. In contrast, ExT did not restore the reduced GABA _A receptor-mediated tonic inhibitory current in HF rats. A GABA _A receptor blocker (bicuculline, 20 μM) increased the firing rate in HF rats with ExT (P < 0.01) but not in those without ExT. Collectively, these results show that ExT normalized the elevated firing activity by increasing synaptic GABA release in PVN-RVLM neurons in HF rats. Our findings provide a brain mechanism underlying the beneficial effects of ExT in HF, which may shed light on the pathophysiology of other diseases accompanied by sympathetic hyperactivation.