Abstract
Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. A neutralizing epitope of porcine epidemic diarrhea virus (PEDV) gene containing SEKDEL was expressed in potato using Agrobacterium-mediated transformation system. Putative transgenic plants were regenerated, and genomic PCR confirmed the presence of PEDV epitope gene in the potato plants. Based on the ELISA results, epitope of PEDV protein made up approximately 0.1% of the total soluble tuber protein.
| Original language | English |
|---|---|
| Pages (from-to) | 125-130 |
| Number of pages | 6 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 82 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2005.08 |
Keywords
- Neutralizing epitope
- Plant expression
- Porcine epidemic
- Porcine epidemic diarrhea virus
- Transgenic potato
Quacquarelli Symonds(QS) Subject Topics
- Agriculture & Forestry
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