Fabrication and application of multilayered polyelectrolyte surfaces for gene delivery

Various gene transfer play important roles in the development of the controlled release of nucleic acid. In this review, we describe a relatively new approach to the immobilization and release of DNA from surfaces and detailed overview of the state of the art. First, methods based on the layer-by-layer assembly of thin multilayered films will be discussed. The applications of layer-by-layer assembly to the fabrication of DNA-containing surfaces will be then presented. We believe this approach can contribute potential method for spatial and/or temporal control over the delivery of DNA.