Fabrication and characterization of PLGA scaffold penetrated with demineralized bone solution

We developed the synthetic/natural hybrid scaffolds with poly (lactide-co-glycolide)(PLGA) and solution using DBP(DB solution) for tissue engineering. Composite scaffolds of PLGA scaffold penetrated DB solution (PLGA-pen-DBP) were manufactured by simple method that PLGA scaffolds manufactured by solvent casting/salt leaching method soaked in DB solution. We evaluated pore structure, wettability, water uptake ability and cell viability of two types of scaffolds. Through SEM and FT-IR, it was confirmed that DB solution was put into the pores of PLGA scaffold. Also, the effects of the impregnation of DB solution on PLGA scaffolds were characterized through water uptake ability and cell viability. It seemed that DBP provided proper pore to PLGA scaffold and improved water absorption. A novel scaffold, which PLGA scaffold penetrated with DB solution, might be suitable to apply as tissue engineered scaffold.