Fabrication and characterization of porous PLLA scaffolds with gentamicin sulfate release system

PLLA scaffold loaded with gentamicin sulfate (GS) was prepared by emulsion freeze-drying method for the prevention of infection and the improvement of wettability, i.e., the cell- and tissue-compatibility. GS-loaded PLLA scaffolds were characterized by scanning electron microscopy (SEM), mercury porosimetry and blue dye intrusion, and the GS release pattern was analyzed by high performance liquid chromatography (HPLC). GS-loaded PLLA scaffolds with porosity above 80%, medium pore size ranging from 30 to 57 μm (with larger pore diameters greater than 150 μm), and specific pore area in the range of 35 to 75 (m²/g) were manufactured by varying processing parameter as GS concentration. It was observed that GS-loaded PLLA scaffolds were highly porous with good interconnections between pores for allowing cell adhesion and growth. These scaffolds may be applicable for scaffold as structures that facilitate either tissue regeneration or repair during reconstructive operations.