Fast and efficient microscale radiosynthesis of 3′-deoxy-3′- [¹⁸F]fluorothymidine

Microscale radiosynthesis on a miniaturized device has been reported as a promising tool for positron emission tomography. Here, we studied miscroscale-level radiosynthetic conditions by examining several microscale reactions that affect the radiochemical yield of 3′-deoxy-3′-[ ¹⁸F]fluorothymidine ([¹⁸F]FLT). [¹⁸F]FLT was prepared with 3-N-Boc-5′-O-dimethoxytrityl-3′-O-nosyl-thymidine using the cartridge purification method. [¹⁸F]FLT was prepared with a yield of 71%, and the product's purity was greater than 99%. Our results provide a new guideline for microscale [¹⁸F]FLT radiosynthesis.