Fate of antibiotic resistance genes in mesophilic and thermophilic anaerobic digestion of chemically enhanced primary treatment (CEPT) sludge

Hyun Min Jang; Jingyeong Shin; Sangki Choi; Seung Gu Shin; Ki Young Park; Jinwoo Cho; Young Mo Kim

doi:10.1016/j.biortech.2017.07.153

Fate of antibiotic resistance genes in mesophilic and thermophilic anaerobic digestion of chemically enhanced primary treatment (CEPT) sludge

Hyun Min Jang
, Jingyeong Shin
, Sangki Choi
, Seung Gu Shin
, Ki Young Park
, Jinwoo Cho
, Young Mo Kim^*

^*Corresponding author for this work

Environmental Engineering

Gwangju Institute of Science and Technology
Pohang University of Science and Technology
Konkuk University
Sejong University

Research output: Contribution to journal › Journal article › peer-review

63 Scopus citations

Abstract

Anaerobic digestion (AD) of chemically enhanced primary treatment (CEPT) sludge and non-CEPT (conventional sedimentation) sludge were comparatively operated under mesophilic and thermophilic conditions. The highest methane yield (692.46 ± 0.46 mL CH₄/g VS_removed in CEPT sludge) was observed in mesophilic AD of CEPT sludge. Meanwhile, thermophilic conditions were more favorable for the removal of total antibiotic resistance genes (ARGs). In this study, no measurable difference in the fates and removal of ARGs and class 1 integrin-integrase gene (intI1) was observed between treated non-CEPT and CEPT sludge. However, redundancy analysis indicated that shifts in bacterial community were primarily accountable for the variations in ARGs and intI1. Network analysis further revealed potential host bacteria for ARGs and intI1.

Original language	English
Pages (from-to)	433-444
Number of pages	12
Journal	Bioresource Technology
Volume	244
DOIs	https://doi.org/10.1016/j.biortech.2017.07.153
State	Published - 2017

Keywords

Anaerobic digestion (AD)
Antibiotic resistance genes (ARGs)
Chemically enhanced primary treatment (CEPT)
Microbial community
quantitative PCR (qPCR)

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10.1016/j.biortech.2017.07.153

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title = "Fate of antibiotic resistance genes in mesophilic and thermophilic anaerobic digestion of chemically enhanced primary treatment (CEPT) sludge",

abstract = "Anaerobic digestion (AD) of chemically enhanced primary treatment (CEPT) sludge and non-CEPT (conventional sedimentation) sludge were comparatively operated under mesophilic and thermophilic conditions. The highest methane yield (692.46 ± 0.46 mL CH4/g VSremoved in CEPT sludge) was observed in mesophilic AD of CEPT sludge. Meanwhile, thermophilic conditions were more favorable for the removal of total antibiotic resistance genes (ARGs). In this study, no measurable difference in the fates and removal of ARGs and class 1 integrin-integrase gene (intI1) was observed between treated non-CEPT and CEPT sludge. However, redundancy analysis indicated that shifts in bacterial community were primarily accountable for the variations in ARGs and intI1. Network analysis further revealed potential host bacteria for ARGs and intI1.",

keywords = "Anaerobic digestion (AD), Antibiotic resistance genes (ARGs), Chemically enhanced primary treatment (CEPT), Microbial community, quantitative PCR (qPCR)",

author = "Jang, \{Hyun Min\} and Jingyeong Shin and Sangki Choi and Shin, \{Seung Gu\} and Park, \{Ki Young\} and Jinwoo Cho and Kim, \{Young Mo\}",

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year = "2017",

doi = "10.1016/j.biortech.2017.07.153",

language = "English",

volume = "244",

pages = "433--444",

journal = "Bioresource Technology",

issn = "0960-8524",

}

TY - JOUR

T1 - Fate of antibiotic resistance genes in mesophilic and thermophilic anaerobic digestion of chemically enhanced primary treatment (CEPT) sludge

AU - Jang, Hyun Min

AU - Shin, Jingyeong

AU - Choi, Sangki

AU - Shin, Seung Gu

AU - Park, Ki Young

AU - Cho, Jinwoo

AU - Kim, Young Mo

PY - 2017

Y1 - 2017

N2 - Anaerobic digestion (AD) of chemically enhanced primary treatment (CEPT) sludge and non-CEPT (conventional sedimentation) sludge were comparatively operated under mesophilic and thermophilic conditions. The highest methane yield (692.46 ± 0.46 mL CH4/g VSremoved in CEPT sludge) was observed in mesophilic AD of CEPT sludge. Meanwhile, thermophilic conditions were more favorable for the removal of total antibiotic resistance genes (ARGs). In this study, no measurable difference in the fates and removal of ARGs and class 1 integrin-integrase gene (intI1) was observed between treated non-CEPT and CEPT sludge. However, redundancy analysis indicated that shifts in bacterial community were primarily accountable for the variations in ARGs and intI1. Network analysis further revealed potential host bacteria for ARGs and intI1.

AB - Anaerobic digestion (AD) of chemically enhanced primary treatment (CEPT) sludge and non-CEPT (conventional sedimentation) sludge were comparatively operated under mesophilic and thermophilic conditions. The highest methane yield (692.46 ± 0.46 mL CH4/g VSremoved in CEPT sludge) was observed in mesophilic AD of CEPT sludge. Meanwhile, thermophilic conditions were more favorable for the removal of total antibiotic resistance genes (ARGs). In this study, no measurable difference in the fates and removal of ARGs and class 1 integrin-integrase gene (intI1) was observed between treated non-CEPT and CEPT sludge. However, redundancy analysis indicated that shifts in bacterial community were primarily accountable for the variations in ARGs and intI1. Network analysis further revealed potential host bacteria for ARGs and intI1.

KW - Anaerobic digestion (AD)

KW - Antibiotic resistance genes (ARGs)

KW - Chemically enhanced primary treatment (CEPT)

KW - Microbial community

KW - quantitative PCR (qPCR)

UR - https://www.scopus.com/pages/publications/85026733947

U2 - 10.1016/j.biortech.2017.07.153

DO - 10.1016/j.biortech.2017.07.153

M3 - Journal article

C2 - 28797965

AN - SCOPUS:85026733947

SN - 0960-8524

VL - 244

SP - 433

EP - 444

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Fate of antibiotic resistance genes in mesophilic and thermophilic anaerobic digestion of chemically enhanced primary treatment (CEPT) sludge

Abstract

Keywords

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