Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes

Hye Yeon Jang; Gi Beum Kim; Jeong Mi Kim; Sang Yull Kang; Hyun Jo Youn; Jinny Park; Su Yeon Ro; Eun Yong Chung; Kwang Hyun Park; Jong Suk Kim

doi:10.3390/ijms242417358

Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes

Hye Yeon Jang
, Gi Beum Kim
, Jeong Mi Kim
, Sang Yull Kang
, Hyun Jo Youn
, Jinny Park
, Su Yeon Ro
, Eun Yong Chung
, Kwang Hyun Park^*
, Jong Suk Kim^*

^*Corresponding author for this work

Department of Medicine

Research output: Contribution to journal › Journal article › peer-review

18 Scopus citations

Abstract

Fisetin is a flavonoid found in plants and has been reported to be effective in various human diseases. However, the effective mechanisms of ultraviolet-A (UVA)-mediated skin damage are not yet clear. In this study, we investigated the protective mechanisms of fisetin regarding UVA-induced human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) damages. Fisetin showed a cytoprotective effect against UVA irradiation and suppressed matrix metalloproteinases (MMPs), MMP-1, and MMP-3 expression. In addition, fisetin was rescued, which decreased mRNA levels of pro-inflammatory cytokines, reactive oxygen species production, and the downregulation of MAPK/AP-1 related protein and NADPH oxidase (NOX) mRNA levels. Furthermore, UVA-induced MMP-1 and MMP-3 were effectively inhibited by siRNAs to NOX 1 to 5 in HDFs and HEKs. These results indicate that fisetin suppresses UVA-induced damage through the NOX/ROS/MAPK pathway in HDFs and HEKs.

Original language	English
Article number	17358
Journal	International Journal of Molecular Sciences
Volume	24
Issue number	24
DOIs	https://doi.org/10.3390/ijms242417358
State	Published - 2023.12

Keywords

fisetin
matrix metalloproteinase
NADPH oxidase
reactive oxygen stress
ultraviolet A

Quacquarelli Symonds(QS) Subject Topics

Computer Science & Information Systems
Engineering - Petroleum
Data Science
Engineering - Chemical
Chemistry
Biological Sciences

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10.3390/ijms242417358

Cite this

Jang, H. Y., Kim, G. B., Kim, J. M., Kang, S. Y., Youn, H. J., Park, J., Ro, S. Y., Chung, E. Y., Park, K. H., & Kim, J. S. (2023). Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes. International Journal of Molecular Sciences, 24(24), Article 17358. https://doi.org/10.3390/ijms242417358

@article{12e35f44029548caa07a33fa25f3e244,

title = "Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes",

abstract = "Fisetin is a flavonoid found in plants and has been reported to be effective in various human diseases. However, the effective mechanisms of ultraviolet-A (UVA)-mediated skin damage are not yet clear. In this study, we investigated the protective mechanisms of fisetin regarding UVA-induced human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) damages. Fisetin showed a cytoprotective effect against UVA irradiation and suppressed matrix metalloproteinases (MMPs), MMP-1, and MMP-3 expression. In addition, fisetin was rescued, which decreased mRNA levels of pro-inflammatory cytokines, reactive oxygen species production, and the downregulation of MAPK/AP-1 related protein and NADPH oxidase (NOX) mRNA levels. Furthermore, UVA-induced MMP-1 and MMP-3 were effectively inhibited by siRNAs to NOX 1 to 5 in HDFs and HEKs. These results indicate that fisetin suppresses UVA-induced damage through the NOX/ROS/MAPK pathway in HDFs and HEKs.",

keywords = "fisetin, matrix metalloproteinase, NADPH oxidase, reactive oxygen stress, ultraviolet A",

author = "Jang, \{Hye Yeon\} and Kim, \{Gi Beum\} and Kim, \{Jeong Mi\} and Kang, \{Sang Yull\} and Youn, \{Hyun Jo\} and Jinny Park and Ro, \{Su Yeon\} and Chung, \{Eun Yong\} and Park, \{Kwang Hyun\} and Kim, \{Jong Suk\}",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = dec,

doi = "10.3390/ijms242417358",

language = "English",

volume = "24",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

number = "24",

}

TY - JOUR

T1 - Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes

AU - Jang, Hye Yeon

AU - Kim, Gi Beum

AU - Kim, Jeong Mi

AU - Kang, Sang Yull

AU - Youn, Hyun Jo

AU - Park, Jinny

AU - Ro, Su Yeon

AU - Chung, Eun Yong

AU - Park, Kwang Hyun

AU - Kim, Jong Suk

PY - 2023/12

Y1 - 2023/12

N2 - Fisetin is a flavonoid found in plants and has been reported to be effective in various human diseases. However, the effective mechanisms of ultraviolet-A (UVA)-mediated skin damage are not yet clear. In this study, we investigated the protective mechanisms of fisetin regarding UVA-induced human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) damages. Fisetin showed a cytoprotective effect against UVA irradiation and suppressed matrix metalloproteinases (MMPs), MMP-1, and MMP-3 expression. In addition, fisetin was rescued, which decreased mRNA levels of pro-inflammatory cytokines, reactive oxygen species production, and the downregulation of MAPK/AP-1 related protein and NADPH oxidase (NOX) mRNA levels. Furthermore, UVA-induced MMP-1 and MMP-3 were effectively inhibited by siRNAs to NOX 1 to 5 in HDFs and HEKs. These results indicate that fisetin suppresses UVA-induced damage through the NOX/ROS/MAPK pathway in HDFs and HEKs.

AB - Fisetin is a flavonoid found in plants and has been reported to be effective in various human diseases. However, the effective mechanisms of ultraviolet-A (UVA)-mediated skin damage are not yet clear. In this study, we investigated the protective mechanisms of fisetin regarding UVA-induced human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) damages. Fisetin showed a cytoprotective effect against UVA irradiation and suppressed matrix metalloproteinases (MMPs), MMP-1, and MMP-3 expression. In addition, fisetin was rescued, which decreased mRNA levels of pro-inflammatory cytokines, reactive oxygen species production, and the downregulation of MAPK/AP-1 related protein and NADPH oxidase (NOX) mRNA levels. Furthermore, UVA-induced MMP-1 and MMP-3 were effectively inhibited by siRNAs to NOX 1 to 5 in HDFs and HEKs. These results indicate that fisetin suppresses UVA-induced damage through the NOX/ROS/MAPK pathway in HDFs and HEKs.

KW - fisetin

KW - matrix metalloproteinase

KW - NADPH oxidase

KW - reactive oxygen stress

KW - ultraviolet A

UR - https://www.scopus.com/pages/publications/85180668782

U2 - 10.3390/ijms242417358

DO - 10.3390/ijms242417358

M3 - Journal article

C2 - 38139186

AN - SCOPUS:85180668782

SN - 1661-6596

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 24

M1 - 17358

ER -

Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes

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Research from Jeonbuk National University Medical School Provides New Data on Molecular Science (Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal ...)

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Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

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Research from Jeonbuk National University Medical School Provides New Data on Molecular Science (Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal ...)

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