Flavobacterium sangjuense sp. nov. isolated from sediment

A yellow-pigmented bacterial strain, GS03^T, was isolated from sediment in a branch of the Nackdong River in Sangju, Korea. Cells were observed to be Gram-negative, aerobic and rod-shaped with gliding motility, and to be positive for catalase and oxidase. Growth was found to occur at 4–30 °C (optimum 25 °C), at pH 7.0–8.5 (optimum pH 7.5) and at NaCl 0% (optimum NaCl 0%, w/v). The major cellular fatty acids (> 10% of the total) were identified as iso C_15:0, iso C_15:1 G, C_15:1ω6c, iso C_15: 0 3-OH and iso C_17: 0 3-OH. The major respiratory quinone was found to be menaquinone MK-6. The genome sequence of GS03^T is 3.1 Mb with G+C content of 36.1 mol%. The major polar lipids of the isolate were identified as phosphatidylethanolamine, three unidentified aminolipids, two unidentified phospholipids, an unidentified lipid and an unidentified aminophospholipid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GS03^T clusters with Flavobacterium paronense KNUS1T^T, with similarity of 96.8%. The phenotypic, phylogenetic, and chemotaxonomic characteristics indicate that strain GS03^T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium sangjuense sp. nov. is proposed. The type strain is GS03^T (= FBCC 502459^T = KCTC 62568^T = JCM 32764^T).