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Flow cytometry and lineage tracing study for identification of adipocyte precursor cell (APC) populations

  • Ju Hee Lee
  • , Azadeh Yeganeh
  • , Hisato Konoeda
  • , Joon Ho Moon
  • , Hoon Ki Sung*
  • *Corresponding author for this work
  • University of Toronto

Research output: Contribution to conferenceChapterpeer-review

Abstract

Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immunophenotyping, quantification, and isolation of distinct populations, which is critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of adipocyte precursor cells using flow cytometry and FACS, defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the plasticity and the cellular origin of APCs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-121
Number of pages11
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1752
ISSN (Print)1064-3745

Keywords

  • Adipocyte precursor cells (APCs)
  • Adipose tissue biology
  • Cre-recombinase transgenic mouse model
  • Flow cytometry
  • Lineage tracing

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