G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β₁- and β₂-adrenergic receptors

β₁- and β₂-adrenergic receptors (ARs) regulate cardiac contractility, calcium handling and protein phosphorylation. The present study aimed to examine the expression levels of vascular endothelial growth factor A (VEGF-A) and several G proteins, and the phosphorylation of transcription factor GATA binding protein 4(GATA4), by western blot analysis, using isolated hearts from 6 month-old transgenic (TG) mice that overexpress β₁AR or β₂AR. Cardiac contractility/relaxation and heart rate was increased in both β₁AR TG and β₂AR TG mouse hearts compared with wild type; however, no signifiicant differences were observed between the β₁- and β₂AR TG mouse hearts. Protein expression levels of inhibitory guanine nucleotide-binding protein (Gi) 2, Gi3 and G-protein-coupled receptor kinase 2 were upregulated in both TG mice, although the upregulation of Gi2 was more prominent in the β₂AR TG mice. VEGF-A expression levels were also increased in both TG mice, and were highest in the β₁AR TG mice. In addition, the levels of phosphorylated-GATA4 expression were increased in β₁- and β₂AR TG mice. In conclusion, the present study demonstrated that cardiac contractility/relaxation and heart rate is increased in β₁AR TG and β₂AR TG mice, and indicated that this increase may be related to the overexpression of G proteins and G-protein-associated proteins.