Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier:in vitro transfection

In Kyu Park; Hu Lin Jiang; Seung Eun Cook; Myung Haing Cho; Su ll Kim; Hwan Jeong Jeong; Toshihiro Akaike; Chong Su Cho

doi:10.1007/BF02975895

Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier:in vitro transfection

In Kyu Park^*
, Hu Lin Jiang^*
, Seung Eun Cook^*
, Myung Haing Cho^*
, Su ll Kim^*
, Hwan Jeong Jeong^*
, Toshihiro Akaike^*
, Chong Su Cho^*

^*Corresponding author for this work

Department of Medicine

Seoul National University
Wonkwang University
Institute of Science Tokyo

Research output: Contribution to journal › Journal article › peer-review

29 Scopus citations

Abstract

Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca²⁺ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.

Original language	English
Pages (from-to)	1284-1289
Number of pages	6
Journal	Archives of Pharmacal Research
Volume	27
Issue number	12
DOIs	https://doi.org/10.1007/BF02975895
State	Published - 2004.12

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Galactosylated chitosan
Gene delivery
Hepatocyte
Poly(vinyl pyrrolidone)

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10.1007/BF02975895

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title = "Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier:in vitro transfection",

abstract = "Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca2+ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.",

keywords = "Galactosylated chitosan, Gene delivery, Hepatocyte, Poly(vinyl pyrrolidone)",

author = "Park, \{In Kyu\} and Jiang, \{Hu Lin\} and Cook, \{Seung Eun\} and Cho, \{Myung Haing\} and Kim, \{Su ll\} and Jeong, \{Hwan Jeong\} and Toshihiro Akaike and Cho, \{Chong Su\}",

note = "Publisher Copyright: {\textcopyright} 2004, The Pharmaceutical Society of Korea.",

year = "2004",

month = dec,

doi = "10.1007/BF02975895",

language = "English",

volume = "27",

pages = "1284--1289",

journal = "Archives of Pharmacal Research",

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TY - JOUR

T1 - Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier:in vitro transfection

AU - Park, In Kyu

AU - Jiang, Hu Lin

AU - Cook, Seung Eun

AU - Cho, Myung Haing

AU - Kim, Su ll

AU - Jeong, Hwan Jeong

AU - Akaike, Toshihiro

AU - Cho, Chong Su

PY - 2004/12

Y1 - 2004/12

N2 - Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca2+ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.

AB - Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca2+ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.

KW - Galactosylated chitosan

KW - Gene delivery

KW - Hepatocyte

KW - Poly(vinyl pyrrolidone)

UR - https://www.scopus.com/pages/publications/13744260137

U2 - 10.1007/BF02975895

DO - 10.1007/BF02975895

M3 - Journal article

C2 - 15648108

AN - SCOPUS:13744260137

SN - 0253-6269

VL - 27

SP - 1284

EP - 1289

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 12

ER -

Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier:in vitro transfection

Abstract

UN SDGs

Keywords

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