Glucosylated polypropylenimine dendrimer as a novel gene carrier

You Kyoung Kim; In Kyu Park; Hu Lin Jiang; Rohidas Arote; Hwan Jeong; Eun Mi Kim; Myung Haing Cho; Hee Seung Bom; Ng Su Cho

doi:10.4028/0-87849-436-7.457

Glucosylated polypropylenimine dendrimer as a novel gene carrier

You Kyoung Kim^*
, In Kyu Park
, Hu Lin Jiang
, Rohidas Arote
, Hwan Jeong
, Eun Mi Kim
, Myung Haing Cho
, Hee Seung Bom
, Ng Su Cho

^*Corresponding author for this work

Department of Medicine

Seoul National University
Jeonbuk National University
Chonnam National University

Research output: Contribution to journal › Journal article › peer-review

5 Scopus citations

Abstract

Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micro-method. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.

Original language	English
Pages (from-to)	457-460
Number of pages	4
Journal	Key Engineering Materials
Volume	342-343
DOIs	https://doi.org/10.4028/0-87849-436-7.457
State	Published - 2007

Keywords

Cytotoxicity
Gene delivery
Glucosylation
Polypropylenimine (PPI) dendrimer

Quacquarelli Symonds(QS) Subject Topics

Engineering - Mechanical
Materials Science

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10.4028/0-87849-436-7.457

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title = "Glucosylated polypropylenimine dendrimer as a novel gene carrier",

abstract = "Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micro-method. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.",

keywords = "Cytotoxicity, Gene delivery, Glucosylation, Polypropylenimine (PPI) dendrimer",

author = "Kim, \{You Kyoung\} and Park, \{In Kyu\} and Jiang, \{Hu Lin\} and Rohidas Arote and Hwan Jeong and Kim, \{Eun Mi\} and Cho, \{Myung Haing\} and Bom, \{Hee Seung\} and Cho, \{Ng Su\}",

year = "2007",

doi = "10.4028/0-87849-436-7.457",

language = "English",

volume = "342-343",

pages = "457--460",

journal = "Key Engineering Materials",

issn = "1013-9826",

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TY - JOUR

T1 - Glucosylated polypropylenimine dendrimer as a novel gene carrier

AU - Kim, You Kyoung

AU - Park, In Kyu

AU - Jiang, Hu Lin

AU - Arote, Rohidas

AU - Jeong, Hwan

AU - Kim, Eun Mi

AU - Cho, Myung Haing

AU - Bom, Hee Seung

AU - Cho, Ng Su

PY - 2007

Y1 - 2007

N2 - Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micro-method. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.

AB - Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micro-method. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.

KW - Cytotoxicity

KW - Gene delivery

KW - Glucosylation

KW - Polypropylenimine (PPI) dendrimer

UR - https://www.scopus.com/pages/publications/34147128292

U2 - 10.4028/0-87849-436-7.457

DO - 10.4028/0-87849-436-7.457

M3 - Journal article

AN - SCOPUS:34147128292

SN - 1013-9826

VL - 342-343

SP - 457

EP - 460

JO - Key Engineering Materials

JF - Key Engineering Materials

ER -

Glucosylated polypropylenimine dendrimer as a novel gene carrier

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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