Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil

A Gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain BH103^T, was isolated from saltern soil in Gomso, Korea. Cells of strain BH103^T were strictly aerobic, motile, straight rods and grew at pH 7.0–10.8 (optimum, pH 8.5), at 10–55 ºC (optimum, 28 ºC) and at salinities of 0–23% (w/v) NaCl (optimum, 14% NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH103^T belongs to the genus Halomonas, showing highest sequence similarity to Halomonas boliviensis LC1^T (97.7 %), Halomonas neptunia Eplume1^T (97.7 %), Halomonas variabilis III^T (97.7 %), Halomonas alkaliantarctica CRSS^T (97.7 %), Halomonas olivaria TYRC17^T (97.5 %), Halomonas titanicae BH1^T (97.2 %) and Halomonas sulfidaeris Esulfide1T (96.2 %). The predominant ubiquinone was Q-9 The major fatty acids were C_{18 : 1}ω7c, C_{16 : 1}ω7c and/or iso-C_{15 : 0} 2-OH, C_{16 : 0} and C_{12 : 0} 3-OH. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and an unknown phospholipid. The DNA G+C content of this novel isolate was 54.7 mol%. DNA–DNA relatedness between strain BH103^T and H. boliviensis KACC 16615^T, H. neptunia KCTC 2888^T, H. variabilis KCTC 2889^T, H. alkaliantarctica KCTC 22844^T, H. olivaria DSM 19074^T, H. titanicae JCM 16411^T and H. sulfidaeris DSM 15722^T was 45, 41, 39, 32, 38, 45 and 35 %, respectively. On the basis of polyphasic analysis from this study, strain BH103^T represents a novel species of the genus Halomonas, for which the name Halomonas salicampi sp. nov. is proposed. The type strain is BH103^T (=KACC 17609^T=NBRC 109914^T=NCAIM B 02528^T).