Abstract
Objective: to investigate whether harmine has a promotive effect on human periodontal ligament cells (hPDLCs)-induced tissue regeneration. Materials and Methods: Various concentrations of harmine on hPDLCs proliferation were tested. Osteogenic and cementogenic characteristics were examined in hPDLC/rhBMP-2 and hPDLC/harmine by alizarin red S staining, real-time PCR, and Western blotting assay. The activity of harmine was investigated in an ectopic transplantation nude mouse model. Results: We determined that 10 μM of harmine was the threshold concentration. hPDLC/harmine showed similar mineralized nodule formation in alizarin S staining compared to hPDLC/rhBMP-2. In real-time PCR, the highest gene expression level was observed for Runx2 in hPDLC/harmine at all time points. The level of CEMP-1 in hPDLC/harmine was higher at 7 days than hPDLCs alone. Thicker band of Runx2 in hPDLC/harmine was observed than in hPDLC/rhBMP-2 at 7 days by Western blotting. The band for CEMP-1 in hPDLC/harmine was thicker than hPDLCs alone at both 7 and 14 days. In ectopic transplantation, hPDLCs with harmine showed a comparable amount of mineralized tissue formation compared to rhBMP-2. hPDLCs with harmine or rhBMP-2 formed both bone and cementum-like tissue with Sharpey's fiber-like collagen insertion. Conclusion: Harmine can be a potential candidate for promoting hPDLCs-induced tissue regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 456-464 |
| Number of pages | 9 |
| Journal | Oral Diseases |
| Volume | 24 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2018.04 |
Keywords
- harmine
- periodontal ligament
- periodontium
- regenerative medicine
- stem cell
Quacquarelli Symonds(QS) Subject Topics
- Dentistry
- Medicine
Fingerprint
Dive into the research topics of 'Harmine promotes periodontal ligament cell-induced tissue regeneration'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver