Hepatocyte growth factor upregulates thymosin β4 in human umbilical vein endothelial cells

In Suk Oh; Sang Sup So; Kwang Yeop Jahng; Hwan Gyu Kim

doi:10.1016/S0006-291X(02)00888-4

Hepatocyte growth factor upregulates thymosin β4 in human umbilical vein endothelial cells

In Suk Oh
, Sang Sup So
, Kwang Yeop Jahng
, Hwan Gyu Kim

Department of Biological Sciences

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

21 Scopus citations

Abstract

Hepatocyte growth factor (HGF) is a mesenchymal-derived cytokine. It exerts in vitro a motogenic effect on various target cells, which is displayed either by cell scattering, locomotion, and migration during the wound repair process of cultured cells, or invasiveness through the extracellular matrix. Although it is known that HGF influences the motogenic effect of endothelial cells, the precise effects of HGF during angiogenesis are still poorly understood. To identify genes regulated via HGF signaling in HUVECs, we used the differential display polymerase chain reaction. In this study, thymosin β4 was found to be differentially expressed in HGF-treated HUVECs compared with control. Data from HPLC profile and induction of MMPs indicate that HGF may affect the biological behavior of HUVECs through a combination of the direct effects of HGF itself and indirect effects mediated via induction of thymosin β4 in vitro.

Original language	English
Pages (from-to)	401-405
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	296
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(02)00888-4
State	Published - 2002

Keywords

DD-PCR
HGF
HUVECs
MMP-2
Thymosin β4

Quacquarelli Symonds(QS) Subject Topics

Biological Sciences

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10.1016/S0006-291X(02)00888-4

Cite this

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title = "Hepatocyte growth factor upregulates thymosin β4 in human umbilical vein endothelial cells",

abstract = "Hepatocyte growth factor (HGF) is a mesenchymal-derived cytokine. It exerts in vitro a motogenic effect on various target cells, which is displayed either by cell scattering, locomotion, and migration during the wound repair process of cultured cells, or invasiveness through the extracellular matrix. Although it is known that HGF influences the motogenic effect of endothelial cells, the precise effects of HGF during angiogenesis are still poorly understood. To identify genes regulated via HGF signaling in HUVECs, we used the differential display polymerase chain reaction. In this study, thymosin β4 was found to be differentially expressed in HGF-treated HUVECs compared with control. Data from HPLC profile and induction of MMPs indicate that HGF may affect the biological behavior of HUVECs through a combination of the direct effects of HGF itself and indirect effects mediated via induction of thymosin β4 in vitro.",

keywords = "DD-PCR, HGF, HUVECs, MMP-2, Thymosin β4",

author = "Oh, \{In Suk\} and So, \{Sang Sup\} and Jahng, \{Kwang Yeop\} and Kim, \{Hwan Gyu\}",

year = "2002",

doi = "10.1016/S0006-291X(02)00888-4",

language = "English",

volume = "296",

pages = "401--405",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

number = "2",

}

TY - JOUR

T1 - Hepatocyte growth factor upregulates thymosin β4 in human umbilical vein endothelial cells

AU - Oh, In Suk

AU - So, Sang Sup

AU - Jahng, Kwang Yeop

AU - Kim, Hwan Gyu

PY - 2002

Y1 - 2002

N2 - Hepatocyte growth factor (HGF) is a mesenchymal-derived cytokine. It exerts in vitro a motogenic effect on various target cells, which is displayed either by cell scattering, locomotion, and migration during the wound repair process of cultured cells, or invasiveness through the extracellular matrix. Although it is known that HGF influences the motogenic effect of endothelial cells, the precise effects of HGF during angiogenesis are still poorly understood. To identify genes regulated via HGF signaling in HUVECs, we used the differential display polymerase chain reaction. In this study, thymosin β4 was found to be differentially expressed in HGF-treated HUVECs compared with control. Data from HPLC profile and induction of MMPs indicate that HGF may affect the biological behavior of HUVECs through a combination of the direct effects of HGF itself and indirect effects mediated via induction of thymosin β4 in vitro.

AB - Hepatocyte growth factor (HGF) is a mesenchymal-derived cytokine. It exerts in vitro a motogenic effect on various target cells, which is displayed either by cell scattering, locomotion, and migration during the wound repair process of cultured cells, or invasiveness through the extracellular matrix. Although it is known that HGF influences the motogenic effect of endothelial cells, the precise effects of HGF during angiogenesis are still poorly understood. To identify genes regulated via HGF signaling in HUVECs, we used the differential display polymerase chain reaction. In this study, thymosin β4 was found to be differentially expressed in HGF-treated HUVECs compared with control. Data from HPLC profile and induction of MMPs indicate that HGF may affect the biological behavior of HUVECs through a combination of the direct effects of HGF itself and indirect effects mediated via induction of thymosin β4 in vitro.

KW - DD-PCR

KW - HGF

KW - HUVECs

KW - MMP-2

KW - Thymosin β4

UR - https://www.scopus.com/pages/publications/0036386975

U2 - 10.1016/S0006-291X(02)00888-4

DO - 10.1016/S0006-291X(02)00888-4

M3 - Journal article

C2 - 12163032

AN - SCOPUS:0036386975

SN - 0006-291X

VL - 296

SP - 401

EP - 405

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Hepatocyte growth factor upregulates thymosin β4 in human umbilical vein endothelial cells

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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