Heterologous expression of endo-1,4-beta-xylanasea from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy; Saravanakumar Thiyagarajan; Yu Lim Son; Seung Moon Park

doi:10.4489/MYCO.2011.39.2.121

Heterologous expression of endo-1,4-beta-xylanasea from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy
, Saravanakumar Thiyagarajan
, Yu Lim Son
, Seung Moon Park^*

^*Corresponding author for this work

Department of Bioenvironmental Chemistry

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

3 Scopus citations

Abstract

The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.

Original language	English
Pages (from-to)	121-124
Number of pages	4
Journal	Mycobiology
Volume	39
Issue number	2
DOIs	https://doi.org/10.4489/MYCO.2011.39.2.121
State	Published - 2011.06

Keywords

Hemicellulose
Phanerochaete chsysosporium
Pichia pastoris
Xylan
Xylanase

Quacquarelli Symonds(QS) Subject Topics

Medicine
Biological Sciences

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10.4489/MYCO.2011.39.2.121

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title = "Heterologous expression of endo-1,4-beta-xylanasea from Phanerochaete chrysosporium in Pichia pastoris",

abstract = "The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.",

keywords = "Hemicellulose, Phanerochaete chsysosporium, Pichia pastoris, Xylan, Xylanase",

author = "Huy, \{Nguyen Duc\} and Saravanakumar Thiyagarajan and Son, \{Yu Lim\} and Park, \{Seung Moon\}",

year = "2011",

month = jun,

doi = "10.4489/MYCO.2011.39.2.121",

language = "English",

volume = "39",

pages = "121--124",

journal = "Mycobiology",

issn = "1229-8093",

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T1 - Heterologous expression of endo-1,4-beta-xylanasea from Phanerochaete chrysosporium in Pichia pastoris

AU - Huy, Nguyen Duc

AU - Thiyagarajan, Saravanakumar

AU - Son, Yu Lim

AU - Park, Seung Moon

PY - 2011/6

Y1 - 2011/6

N2 - The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.

AB - The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.

KW - Hemicellulose

KW - Phanerochaete chsysosporium

KW - Pichia pastoris

KW - Xylan

KW - Xylanase

UR - https://www.scopus.com/pages/publications/84863078859

U2 - 10.4489/MYCO.2011.39.2.121

DO - 10.4489/MYCO.2011.39.2.121

M3 - Journal article

AN - SCOPUS:84863078859

SN - 1229-8093

VL - 39

SP - 121

EP - 124

JO - Mycobiology

JF - Mycobiology

IS - 2

ER -

Heterologous expression of endo-1,4-beta-xylanasea from Phanerochaete chrysosporium in Pichia pastoris

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

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