Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy; Seung Wook Kim; Seung Moon Park

doi:10.1016/j.jbiosc.2011.02.010

Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Nguyen Duc Huy
, Seung Wook Kim
, Seung Moon Park^*

^*Corresponding author for this work

Department of Bioenvironmental Chemistry

Jeonbuk National University
Korea University

Research output: Contribution to journal › Journal article › peer-review

14 Scopus citations

Abstract

The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l^-1.

Original language	English
Pages (from-to)	654-657
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	111
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2011.02.010
State	Published - 2011.06

Keywords

Glycoside hydrolase
Phanerochaete chrysosporium
Pichia pastoris
Xylan
Xylanase

Quacquarelli Symonds(QS) Subject Topics

Engineering - Chemical
Biological Sciences

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10.1016/j.jbiosc.2011.02.010

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title = "Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris",

abstract = "The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.",

keywords = "Glycoside hydrolase, Phanerochaete chrysosporium, Pichia pastoris, Xylan, Xylanase",

author = "Huy, \{Nguyen Duc\} and Kim, \{Seung Wook\} and Park, \{Seung Moon\}",

year = "2011",

month = jun,

doi = "10.1016/j.jbiosc.2011.02.010",

language = "English",

volume = "111",

pages = "654--657",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

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TY - JOUR

T1 - Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

AU - Huy, Nguyen Duc

AU - Kim, Seung Wook

AU - Park, Seung Moon

PY - 2011/6

Y1 - 2011/6

N2 - The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.

AB - The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500U.l-1.

KW - Glycoside hydrolase

KW - Phanerochaete chrysosporium

KW - Pichia pastoris

KW - Xylan

KW - Xylanase

UR - https://www.scopus.com/pages/publications/79957616856

U2 - 10.1016/j.jbiosc.2011.02.010

DO - 10.1016/j.jbiosc.2011.02.010

M3 - Journal article

C2 - 21393056

AN - SCOPUS:79957616856

SN - 1389-1723

VL - 111

SP - 654

EP - 657

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 6

ER -

Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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