Heterologous expression of phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green

Yu Lim Son; Hyoun Young Kim; Saravanakumar Thiyagarajan; Jing Jing Xu; Seung Moon Park

doi:10.5941/MYCO.2012.40.4.258

Heterologous expression of phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green

Yu Lim Son
, Hyoun Young Kim
, Saravanakumar Thiyagarajan
, Jing Jing Xu
, Seung Moon Park^*

^*Corresponding author for this work

Department of Bioenvironmental Chemistry

Jeonbuk National University
Institute of Sunchang Fermented Soybean Products

Research output: Contribution to journal › Journal article › peer-review

8 Scopus citations

Abstract

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H₂O₂ over 7.0nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H₂O₂ improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150μM within 90min.

Original language	English
Pages (from-to)	258-262
Number of pages	5
Journal	Mycobiology
Volume	40
Issue number	4
DOIs	https://doi.org/10.5941/MYCO.2012.40.4.258
State	Published - 2012

Keywords

Dye decolorization
Glyoxal oxidase
Manganese peroxidase
Phanerochaete chrysosporium
Pichia pastoris

Quacquarelli Symonds(QS) Subject Topics

Medicine
Biological Sciences

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10.5941/MYCO.2012.40.4.258

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title = "Heterologous expression of phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green",

abstract = "cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150μM within 90min.",

keywords = "Dye decolorization, Glyoxal oxidase, Manganese peroxidase, Phanerochaete chrysosporium, Pichia pastoris",

author = "Son, \{Yu Lim\} and Kim, \{Hyoun Young\} and Saravanakumar Thiyagarajan and Xu, \{Jing Jing\} and Park, \{Seung Moon\}",

year = "2012",

doi = "10.5941/MYCO.2012.40.4.258",

language = "English",

volume = "40",

pages = "258--262",

journal = "Mycobiology",

issn = "1229-8093",

number = "4",

}

TY - JOUR

T1 - Heterologous expression of phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green

AU - Son, Yu Lim

AU - Kim, Hyoun Young

AU - Thiyagarajan, Saravanakumar

AU - Xu, Jing Jing

AU - Park, Seung Moon

PY - 2012

Y1 - 2012

N2 - cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150μM within 90min.

AB - cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150μM within 90min.

KW - Dye decolorization

KW - Glyoxal oxidase

KW - Manganese peroxidase

KW - Phanerochaete chrysosporium

KW - Pichia pastoris

UR - https://www.scopus.com/pages/publications/84875164588

U2 - 10.5941/MYCO.2012.40.4.258

DO - 10.5941/MYCO.2012.40.4.258

M3 - Journal article

AN - SCOPUS:84875164588

SN - 1229-8093

VL - 40

SP - 258

EP - 262

JO - Mycobiology

JF - Mycobiology

IS - 4

ER -

Heterologous expression of phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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