@inbook{3dd258417e4b47929d1b776b4e094ea8,
title = "Identification of RNA–DNA Hybrids Associated with R-Loops at the IgH Switch Sequence in Activated B Cells",
abstract = "During transcription and replication, R-loops that contain RNA–DNA hybrids are generated across numerous genomic loci and contribute to many biological events. Using S9.6, a monoclonal antibody against RNA–DNA hybrids, accelerated the study of R-loop biology. An outpouring of recent studies has implicated various contributions of R-loop in physiological cellular functions. Earlier studies using nondenaturing sodium bisulfite probing also supported existence of DNA–RNA hybrids formation in mammalian cells. In activated B cells, RNA–DNA hybrids formation at IgH gene locus of B cells is crucial for class switch recombination that ensure the proper effector function of the antibody. Here, we describe the identification of R-loops associated with the IgH locus using RNA–DNA hybrid immunoprecipitation sequencing and nondenaturing sodium bisulfite probing. This will be helpful for future studies of R-loops status on whole genome as well as on IgH locus in B cells.",
keywords = "B cells, Class switch recombination, DRIP-seq, Nondenaturing sodium bisulfite probing",
author = "Junghyun Lim and Brice Laffleur and Uttiya Basu and Kefei Yu",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2477-7\_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "55--66",
booktitle = "Methods in Molecular Biology",
}