Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation

Lian Hua Luo; Pil Soo Seo; Jeong Woo Seo; Sun Yeon Heo; Dae Hyuk Kim; Chul Ho Kim

doi:10.1007/s10529-009-0092-4

Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation

Lian Hua Luo
, Pil Soo Seo
, Jeong Woo Seo
, Sun Yeon Heo
, Dae Hyuk Kim
, Chul Ho Kim

Department of Molecular Biology

Korea Research Institute of Bioscience and Biotechnology
Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

51 Scopus citations

Abstract

To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.

Original language	English
Pages (from-to)	1867-1871
Number of pages	5
Journal	Biotechnology Letters
Volume	31
Issue number	12
DOIs	https://doi.org/10.1007/s10529-009-0092-4
State	Published - 2009.11

Keywords

Desaturase
Escherichia coli
Ethanol tolerance
FabA
Fatty acids composition

Quacquarelli Symonds(QS) Subject Topics

Engineering - Chemical
Biological Sciences

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10.1007/s10529-009-0092-4

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title = "Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation",

abstract = "To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.",

keywords = "Desaturase, Escherichia coli, Ethanol tolerance, FabA, Fatty acids composition",

author = "Luo, \{Lian Hua\} and Seo, \{Pil Soo\} and Seo, \{Jeong Woo\} and Heo, \{Sun Yeon\} and Kim, \{Dae Hyuk\} and Kim, \{Chul Ho\}",

year = "2009",

month = nov,

doi = "10.1007/s10529-009-0092-4",

language = "English",

volume = "31",

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journal = "Biotechnology Letters",

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T1 - Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation

AU - Luo, Lian Hua

AU - Seo, Pil Soo

AU - Seo, Jeong Woo

AU - Heo, Sun Yeon

AU - Kim, Dae Hyuk

AU - Kim, Chul Ho

PY - 2009/11

Y1 - 2009/11

N2 - To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.

AB - To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.

KW - Desaturase

KW - Escherichia coli

KW - Ethanol tolerance

KW - FabA

KW - Fatty acids composition

UR - https://www.scopus.com/pages/publications/73949105851

U2 - 10.1007/s10529-009-0092-4

DO - 10.1007/s10529-009-0092-4

M3 - Journal article

C2 - 19685209

AN - SCOPUS:73949105851

SN - 0141-5492

VL - 31

SP - 1867

EP - 1871

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 12

ER -

Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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