In-depth examination of PrPSc in Holstein cattle carrying the E211K somatic mutation of the bovine prion protein gene (PRNP)

Yong Chan Kim; Kyung Je Park; Ji Yong Hwang; Hoo Chang Park; Hae Eun Kang; Hyun Joo Sohn; Byung Hoon Jeong

doi:10.1111/tbed.14309

In-depth examination of PrP^Sc in Holstein cattle carrying the E211K somatic mutation of the bovine prion protein gene (PRNP)

Yong Chan Kim
, Kyung Je Park
, Ji Yong Hwang
, Hoo Chang Park
, Hae Eun Kang
, Hyun Joo Sohn^*
, Byung Hoon Jeong^*

^*Corresponding author for this work

Korea Zoonosis Research Institute at Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

8 Scopus citations

Abstract

Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrP^Sc) derived from normal prion protein (PrP^C), which is encoded by the prion protein gene (PRNP). We performed an in-depth examination to detect PrP^Sc by using enzyme immunoassay (EIA), real-time quaking-induced conversion reactions (RT-QuIC) and protein misfolding cyclic amplification (PMCA) in nine brain tissues derived from three Holstein cattle carrying the E211K somatic mutation of the bovine PRNP gene. The EIA, RT-QuIC and PMCA analyses were not able to detect the PrP^Sc band in any tested samples. To the best of our knowledge, this report is the first to describe an in-depth examination of PrP^Sc in cattle carrying the E211K somatic mutation of the bovine PRNP gene.

Original language	English
Pages (from-to)	e356-e361
Journal	Transboundary and Emerging Diseases
Volume	69
Issue number	4
DOIs	https://doi.org/10.1111/tbed.14309
State	Published - 2022.07

Keywords

bovine spongiform encephalopathy
E200K
E211K
PMCA
prion
prion disease
PRNP
RT-QuIC
somatic mutation

Quacquarelli Symonds(QS) Subject Topics

Veterinary Science
Biological Sciences

Access to Document

10.1111/tbed.14309

Cite this

@article{50b61444582542ca9eece1bc55f61f20,

title = "In-depth examination of PrPSc in Holstein cattle carrying the E211K somatic mutation of the bovine prion protein gene (PRNP)",

abstract = "Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrPSc) derived from normal prion protein (PrPC), which is encoded by the prion protein gene (PRNP). We performed an in-depth examination to detect PrPSc by using enzyme immunoassay (EIA), real-time quaking-induced conversion reactions (RT-QuIC) and protein misfolding cyclic amplification (PMCA) in nine brain tissues derived from three Holstein cattle carrying the E211K somatic mutation of the bovine PRNP gene. The EIA, RT-QuIC and PMCA analyses were not able to detect the PrPSc band in any tested samples. To the best of our knowledge, this report is the first to describe an in-depth examination of PrPSc in cattle carrying the E211K somatic mutation of the bovine PRNP gene.",

keywords = "bovine spongiform encephalopathy, E200K, E211K, PMCA, prion, prion disease, PRNP, RT-QuIC, somatic mutation",

author = "Kim, \{Yong Chan\} and Park, \{Kyung Je\} and Hwang, \{Ji Yong\} and Park, \{Hoo Chang\} and Kang, \{Hae Eun\} and Sohn, \{Hyun Joo\} and Jeong, \{Byung Hoon\}",

note = "Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH.",

year = "2022",

month = jul,

doi = "10.1111/tbed.14309",

language = "English",

volume = "69",

pages = "e356--e361",

journal = "Transboundary and Emerging Diseases",

issn = "1865-1674",

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T1 - In-depth examination of PrPSc in Holstein cattle carrying the E211K somatic mutation of the bovine prion protein gene (PRNP)

AU - Kim, Yong Chan

AU - Park, Kyung Je

AU - Hwang, Ji Yong

AU - Park, Hoo Chang

AU - Kang, Hae Eun

AU - Sohn, Hyun Joo

AU - Jeong, Byung Hoon

PY - 2022/7

Y1 - 2022/7

N2 - Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrPSc) derived from normal prion protein (PrPC), which is encoded by the prion protein gene (PRNP). We performed an in-depth examination to detect PrPSc by using enzyme immunoassay (EIA), real-time quaking-induced conversion reactions (RT-QuIC) and protein misfolding cyclic amplification (PMCA) in nine brain tissues derived from three Holstein cattle carrying the E211K somatic mutation of the bovine PRNP gene. The EIA, RT-QuIC and PMCA analyses were not able to detect the PrPSc band in any tested samples. To the best of our knowledge, this report is the first to describe an in-depth examination of PrPSc in cattle carrying the E211K somatic mutation of the bovine PRNP gene.

AB - Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrPSc) derived from normal prion protein (PrPC), which is encoded by the prion protein gene (PRNP). We performed an in-depth examination to detect PrPSc by using enzyme immunoassay (EIA), real-time quaking-induced conversion reactions (RT-QuIC) and protein misfolding cyclic amplification (PMCA) in nine brain tissues derived from three Holstein cattle carrying the E211K somatic mutation of the bovine PRNP gene. The EIA, RT-QuIC and PMCA analyses were not able to detect the PrPSc band in any tested samples. To the best of our knowledge, this report is the first to describe an in-depth examination of PrPSc in cattle carrying the E211K somatic mutation of the bovine PRNP gene.

KW - bovine spongiform encephalopathy

KW - E200K

KW - E211K

KW - PMCA

KW - prion

KW - prion disease

KW - PRNP

KW - RT-QuIC

KW - somatic mutation

UR - https://www.scopus.com/pages/publications/85114697239

U2 - 10.1111/tbed.14309

DO - 10.1111/tbed.14309

M3 - Journal article

C2 - 34470082

AN - SCOPUS:85114697239

SN - 1865-1674

VL - 69

SP - e356-e361

JO - Transboundary and Emerging Diseases

JF - Transboundary and Emerging Diseases

IS - 4

ER -