Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages

Jun Hong Park; Sun mee Oh; Soon Sung Lim; Yeon Sil Lee; Hyun Kyung Shin; Yang Seok Oh; Nong Hoon Choe; Jung Han Yoon Park; Jin Kyung Kim

doi:10.1016/j.bbrc.2006.10.008

Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages

Jun Hong Park
, Sun mee Oh
, Soon Sung Lim
, Yeon Sil Lee
, Hyun Kyung Shin
, Yang Seok Oh
, Nong Hoon Choe
, Jung Han Yoon Park
, Jin Kyung Kim^*

^*Corresponding author for this work

Department of Veterinary Medicine

Hallym University
Konkuk University

Research output: Contribution to journal › Journal article › peer-review

50 Scopus citations

Abstract

Foods of plant origin, especially fruits and vegetables, draw increased attention because of their potential benefits to human health. The aim of the present study was to determine in vitro anti-inflammatory activity of four different extracts obtained from the fruits of Rubus coreanus (aqueous and ethanol extracts of unripe and ripe fruits). Among the four extracts, the ethanol extract of unripe fruits of R. coreanus (URCE) suppressed nitric oxide (NO) and prostaglandin E₂ (PGE₂) production in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. We also demonstrated that URCE by itself is a potent inducer of heme oxygenase-1 (HO-1). Inhibition of HO-1 activity by tin protoporphyrin, a specific HO-1 inhibitor, suppressed the URCE-induced reductions in the production of NO and PGE₂ as well as the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). Our data suggest that URCE exerts anti-inflammatory effects in macrophages via activation of the HO-1 pathway and helps to elucidate the mechanism underlying the potential therapeutic value of R. coreanus extracts.

Original language	English
Pages (from-to)	146-152
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	351
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.10.008
State	Published - 2006.12.8

Keywords

Heme oxygenase-1
Inducible nitric oxide synthase
Inflammation
Macrophages
Prostaglandin E
Rubus coreanus

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10.1016/j.bbrc.2006.10.008

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title = "Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages",

abstract = "Foods of plant origin, especially fruits and vegetables, draw increased attention because of their potential benefits to human health. The aim of the present study was to determine in vitro anti-inflammatory activity of four different extracts obtained from the fruits of Rubus coreanus (aqueous and ethanol extracts of unripe and ripe fruits). Among the four extracts, the ethanol extract of unripe fruits of R. coreanus (URCE) suppressed nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. We also demonstrated that URCE by itself is a potent inducer of heme oxygenase-1 (HO-1). Inhibition of HO-1 activity by tin protoporphyrin, a specific HO-1 inhibitor, suppressed the URCE-induced reductions in the production of NO and PGE2 as well as the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). Our data suggest that URCE exerts anti-inflammatory effects in macrophages via activation of the HO-1 pathway and helps to elucidate the mechanism underlying the potential therapeutic value of R. coreanus extracts.",

keywords = "Heme oxygenase-1, Inducible nitric oxide synthase, Inflammation, Macrophages, Prostaglandin E, Rubus coreanus",

author = "Park, \{Jun Hong\} and Oh, \{Sun mee\} and Lim, \{Soon Sung\} and Lee, \{Yeon Sil\} and Shin, \{Hyun Kyung\} and Oh, \{Yang Seok\} and Choe, \{Nong Hoon\} and Park, \{Jung Han Yoon\} and Kim, \{Jin Kyung\}",

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AU - Park, Jun Hong

AU - Oh, Sun mee

AU - Lim, Soon Sung

AU - Lee, Yeon Sil

AU - Shin, Hyun Kyung

AU - Oh, Yang Seok

AU - Choe, Nong Hoon

AU - Park, Jung Han Yoon

AU - Kim, Jin Kyung

PY - 2006/12/8

Y1 - 2006/12/8

N2 - Foods of plant origin, especially fruits and vegetables, draw increased attention because of their potential benefits to human health. The aim of the present study was to determine in vitro anti-inflammatory activity of four different extracts obtained from the fruits of Rubus coreanus (aqueous and ethanol extracts of unripe and ripe fruits). Among the four extracts, the ethanol extract of unripe fruits of R. coreanus (URCE) suppressed nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. We also demonstrated that URCE by itself is a potent inducer of heme oxygenase-1 (HO-1). Inhibition of HO-1 activity by tin protoporphyrin, a specific HO-1 inhibitor, suppressed the URCE-induced reductions in the production of NO and PGE2 as well as the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). Our data suggest that URCE exerts anti-inflammatory effects in macrophages via activation of the HO-1 pathway and helps to elucidate the mechanism underlying the potential therapeutic value of R. coreanus extracts.

AB - Foods of plant origin, especially fruits and vegetables, draw increased attention because of their potential benefits to human health. The aim of the present study was to determine in vitro anti-inflammatory activity of four different extracts obtained from the fruits of Rubus coreanus (aqueous and ethanol extracts of unripe and ripe fruits). Among the four extracts, the ethanol extract of unripe fruits of R. coreanus (URCE) suppressed nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. We also demonstrated that URCE by itself is a potent inducer of heme oxygenase-1 (HO-1). Inhibition of HO-1 activity by tin protoporphyrin, a specific HO-1 inhibitor, suppressed the URCE-induced reductions in the production of NO and PGE2 as well as the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). Our data suggest that URCE exerts anti-inflammatory effects in macrophages via activation of the HO-1 pathway and helps to elucidate the mechanism underlying the potential therapeutic value of R. coreanus extracts.

KW - Heme oxygenase-1

KW - Inducible nitric oxide synthase

KW - Inflammation

KW - Macrophages

KW - Prostaglandin E

KW - Rubus coreanus

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U2 - 10.1016/j.bbrc.2006.10.008

DO - 10.1016/j.bbrc.2006.10.008

M3 - Journal article

C2 - 17049489

AN - SCOPUS:33750376155

SN - 0006-291X

VL - 351

SP - 146

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages

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