KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

Ki Duk Song; Duk Jung Kim; Jong Eun Lee; Cheol Heui Yun; Woon Kyu Lee

doi:10.1016/j.bbrc.2012.02.032

KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

Ki Duk Song
, Duk Jung Kim
, Jong Eun Lee
, Cheol Heui Yun
, Woon Kyu Lee^*

^*Corresponding author for this work

Department of Agricultural Convergence Technology

Seoul National University
The Institute of Hankook Life Science
Yonsei University
Inha University

Research output: Contribution to journal › Journal article › peer-review

29 Scopus citations

Abstract

Krüppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21^WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21^WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21^WAF1/CIP1 transcription.

Original language	English
Pages (from-to)	388-394
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	419
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2012.02.032
State	Published - 2012.03.9

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

DMBA/TPA
Krüppel-like factor 10
P21
Sp1 binding sites
Tumor suppressor

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10.1016/j.bbrc.2012.02.032

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@article{b8a20af7ed4842e89c52a63a170c032a,

title = "KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor",

abstract = "Kr{\"u}ppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21WAF1/CIP1 transcription.",

keywords = "DMBA/TPA, Kr{\"u}ppel-like factor 10, P21, Sp1 binding sites, Tumor suppressor",

author = "Song, \{Ki Duk\} and Kim, \{Duk Jung\} and Lee, \{Jong Eun\} and Yun, \{Cheol Heui\} and Lee, \{Woon Kyu\}",

year = "2012",

month = mar,

day = "9",

doi = "10.1016/j.bbrc.2012.02.032",

language = "English",

volume = "419",

pages = "388--394",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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}

TY - JOUR

T1 - KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

AU - Song, Ki Duk

AU - Kim, Duk Jung

AU - Lee, Jong Eun

AU - Yun, Cheol Heui

AU - Lee, Woon Kyu

PY - 2012/3/9

Y1 - 2012/3/9

N2 - Krüppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21WAF1/CIP1 transcription.

AB - Krüppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21WAF1/CIP1 transcription.

KW - DMBA/TPA

KW - Krüppel-like factor 10

KW - P21

KW - Sp1 binding sites

KW - Tumor suppressor

UR - https://www.scopus.com/pages/publications/84862804986

U2 - 10.1016/j.bbrc.2012.02.032

DO - 10.1016/j.bbrc.2012.02.032

M3 - Journal article

C2 - 22349513

AN - SCOPUS:84862804986

SN - 0006-291X

VL - 419

SP - 388

EP - 394

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

Abstract

UN SDGs

Keywords

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