Molecular analysis of Korean isolate of Barley mild mosaic virus (Iks isolate)

The complete nucleotide sequences of both RNA of an isolated Barley mild mosaic virus (BaMMV) from Iksan, Korea, have been determined. RNA1 was 7273 nucleotides long and encodes for a polyprotein of 2261 amino acids, which contains the eight putative functional proteins. RNA2 was 3520 nucleotides long and encodes for a polyprotein of 894 amino acids, which contains two functional proteins. Results of multiple sequence alignment showed 92.9% similarity with Nal isolate, followed by Sil, UK(F), Asll, Remis M and UK (M) isolates, respectively. Comparison of the BaMMV-Iks polyproteins with the corresponding proteins of BaMMV-Nal isolates showed 95% amino acid sequence identity. The phylogenetic analysis revealed that Iks isolate was closely related to Nal strain and have a common origin.