Abstract
A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased hot pepper plant and its biological and molecular properties were compared to that of PMMoV-J and PMMoV-S. The genomic RNA of PMMoV-Kr consists of 6,356 nucleotides. The nucleotide and amino acid sequences identities of four viral proteins and two noncoding regions among PMMoV-Kr, PMMoV-S and PMMoY-J were 96.9% to 100.0% and 97.5% to 98.6%, respectively. Full-length cDNA amplicon of PMMoV-Kr was directly amplified by RT-PCR with a set of 5'-end primer anchoring T7 RNA promoter sequence and 3'-end virus-specific primer. Capped transcript RNAs from the full-length cDNA clone were highly infectious and caused characteristic symptoms of wild type PMMoV when mechanically inoculated to systemic host plants such as Nicotiana benthamiana and pepper plants.
| Original language | English |
|---|---|
| Pages (from-to) | 361-368 |
| Number of pages | 8 |
| Journal | Plant Pathology Journal |
| Volume | 21 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2005 |
Keywords
- Genome
- Infectious cDNA clone
- Pepper
- Pepper mild mottle virus
- Sequence
- Tobamovirus
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