Mulberry and its main components protect against oxidized low-density lipoprotein-induced endothelial nitric oxide synthase uncoupling

Hwa Young Lee; Mi Ra Oh; Eun Soo Jung; Yang Soo Lee; Deok Su Kim; Seong Sun Kang; Han Jung Chae; Soo Wan Chae

doi:10.1016/j.jff.2016.12.034

Mulberry and its main components protect against oxidized low-density lipoprotein-induced endothelial nitric oxide synthase uncoupling

Hwa Young Lee
, Mi Ra Oh
, Eun Soo Jung
, Yang Soo Lee
, Deok Su Kim
, Seong Sun Kang
, Han Jung Chae^*
, Soo Wan Chae

^*Corresponding author for this work

Jeonbuk National University
Buan Agricultural Development & Technology Center

Research output: Contribution to journal › Journal article › peer-review

9 Scopus citations

Abstract

Nitric oxide (NO), which is produced by endothelial NO synthase (eNOS), is an important protective molecule in the vasculature. However, eNOS coupling and its resultant activation are altered under oxidative stress. Under these conditions, eNOS uses molecular oxygen as a substrate instead of L-arginine, resulting in the production of superoxide anion radicals (O₂^-) rather than NO. We explored the effects of mulberry extract and its components on eNOS uncoupling induced by oxidized low-density lipoprotein in HUVECs and investigated the potential molecular mechanism. Ox-LDL significantly increased O₂^- production and reduced NO release from HUVECs. Pretreatment of cells with mulberry extract and its main components significantly inhibited ox-LDL-induced O₂^- generation, preserving NO level. Mulberry extract also prevented ox-LDL-induced reduction in phospho-eNOS Thr495. These regulatory effects were related to phosphorylation of eNOS Thr495. Mulberry extract and its components can potentially be used to treat ox-LDL-related vascular endothelial dysfunction.

Original language	English
Pages (from-to)	295-302
Number of pages	8
Journal	Journal of Functional Foods
Volume	29
DOIs	https://doi.org/10.1016/j.jff.2016.12.034
State	Published - 2017.02.1

Keywords

Endothelial NOS
Human umbilical vein endothelial cell
Oxidized low-density lipoprotein
Superoxide

Quacquarelli Symonds(QS) Subject Topics

Nursing
Agriculture & Forestry
Medicine

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10.1016/j.jff.2016.12.034

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@article{8be0e726cf714223a3d2d3f3d9cc679d,

title = "Mulberry and its main components protect against oxidized low-density lipoprotein-induced endothelial nitric oxide synthase uncoupling",

abstract = "Nitric oxide (NO), which is produced by endothelial NO synthase (eNOS), is an important protective molecule in the vasculature. However, eNOS coupling and its resultant activation are altered under oxidative stress. Under these conditions, eNOS uses molecular oxygen as a substrate instead of L-arginine, resulting in the production of superoxide anion radicals (O2-) rather than NO. We explored the effects of mulberry extract and its components on eNOS uncoupling induced by oxidized low-density lipoprotein in HUVECs and investigated the potential molecular mechanism. Ox-LDL significantly increased O2- production and reduced NO release from HUVECs. Pretreatment of cells with mulberry extract and its main components significantly inhibited ox-LDL-induced O2- generation, preserving NO level. Mulberry extract also prevented ox-LDL-induced reduction in phospho-eNOS Thr495. These regulatory effects were related to phosphorylation of eNOS Thr495. Mulberry extract and its components can potentially be used to treat ox-LDL-related vascular endothelial dysfunction.",

keywords = "Endothelial NOS, Human umbilical vein endothelial cell, Oxidized low-density lipoprotein, Superoxide",

author = "Lee, \{Hwa Young\} and Oh, \{Mi Ra\} and Jung, \{Eun Soo\} and Lee, \{Yang Soo\} and Kim, \{Deok Su\} and Kang, \{Seong Sun\} and Chae, \{Han Jung\} and Chae, \{Soo Wan\}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

year = "2017",

month = feb,

day = "1",

doi = "10.1016/j.jff.2016.12.034",

language = "English",

volume = "29",

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journal = "Journal of Functional Foods",

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TY - JOUR

T1 - Mulberry and its main components protect against oxidized low-density lipoprotein-induced endothelial nitric oxide synthase uncoupling

AU - Lee, Hwa Young

AU - Oh, Mi Ra

AU - Jung, Eun Soo

AU - Lee, Yang Soo

AU - Kim, Deok Su

AU - Kang, Seong Sun

AU - Chae, Han Jung

AU - Chae, Soo Wan

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Nitric oxide (NO), which is produced by endothelial NO synthase (eNOS), is an important protective molecule in the vasculature. However, eNOS coupling and its resultant activation are altered under oxidative stress. Under these conditions, eNOS uses molecular oxygen as a substrate instead of L-arginine, resulting in the production of superoxide anion radicals (O2-) rather than NO. We explored the effects of mulberry extract and its components on eNOS uncoupling induced by oxidized low-density lipoprotein in HUVECs and investigated the potential molecular mechanism. Ox-LDL significantly increased O2- production and reduced NO release from HUVECs. Pretreatment of cells with mulberry extract and its main components significantly inhibited ox-LDL-induced O2- generation, preserving NO level. Mulberry extract also prevented ox-LDL-induced reduction in phospho-eNOS Thr495. These regulatory effects were related to phosphorylation of eNOS Thr495. Mulberry extract and its components can potentially be used to treat ox-LDL-related vascular endothelial dysfunction.

AB - Nitric oxide (NO), which is produced by endothelial NO synthase (eNOS), is an important protective molecule in the vasculature. However, eNOS coupling and its resultant activation are altered under oxidative stress. Under these conditions, eNOS uses molecular oxygen as a substrate instead of L-arginine, resulting in the production of superoxide anion radicals (O2-) rather than NO. We explored the effects of mulberry extract and its components on eNOS uncoupling induced by oxidized low-density lipoprotein in HUVECs and investigated the potential molecular mechanism. Ox-LDL significantly increased O2- production and reduced NO release from HUVECs. Pretreatment of cells with mulberry extract and its main components significantly inhibited ox-LDL-induced O2- generation, preserving NO level. Mulberry extract also prevented ox-LDL-induced reduction in phospho-eNOS Thr495. These regulatory effects were related to phosphorylation of eNOS Thr495. Mulberry extract and its components can potentially be used to treat ox-LDL-related vascular endothelial dysfunction.

KW - Endothelial NOS

KW - Human umbilical vein endothelial cell

KW - Oxidized low-density lipoprotein

KW - Superoxide

UR - https://www.scopus.com/pages/publications/85009101095

U2 - 10.1016/j.jff.2016.12.034

DO - 10.1016/j.jff.2016.12.034

M3 - Journal article

AN - SCOPUS:85009101095

SN - 1756-4646

VL - 29

SP - 295

EP - 302

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

Mulberry and its main components protect against oxidized low-density lipoprotein-induced endothelial nitric oxide synthase uncoupling

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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