Multiplex fluorophore systems on DNA with new diverse fluorescence properties and ability to sense the hybridization dynamics

We developed a multiplexed fluorophore system on a DNA scaffold (MFD) that produced new and diverse fluorescence properties depending on the mixing pattern and sequence that could not be obtained from each monomer fluorophore. Our approach for producing new fluorescence properties is relatively facile: simply mixing fluorophores on a DNA scaffold provides large variations in the color and intensity using only one excitation wavelength with high “Stokes shifts” (∼190 nm). Furthermore these special fluorescence properties could be controlled by the hybridization pattern and were therefore dependent on the structural changes in DNA.