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Nanotechnology-based Biosensor for the Detection of Harmful Pathogens in Potable Water

  • Francesca Petronella
  • , Melissa De Angelis
  • , Daniela Debiase
  • , Seok Im Lim
  • , Kwang Un Jeong
  • , Nicholas Godman
  • , Dean Evans
  • , Michael McConney
  • , Luciano De Sio
  • National Research Council of Italy
  • University of Rome La Sapienza
  • Jeonbuk National University
  • Air Force Research Laboratory

Research output: Contribution to conferenceConference paperpeer-review

Abstract

We report and discuss our first efforts devoted to realizing an innovative and miniaturized nanotechnology-based biosensor capable of real-time detect and quantify the presence of harmful pathogens dispersed in potable water. Gold nanorods, selected for their colorimetric sensitivity to the refractive index change, are functionalized, via the electrostatic linking method, with anti-Escherichia coli (E.coli) antibody. Selective recognition experiments are performed by utilizing a physiological solution containing different E.coli concentrations, thus exploiting the interaction between E.coli surface antigens and the anti-E.coli antibody. The system is characterized in terms of morphological, optical, and spectroscopic properties. Results reveal an excellent capability to discriminate small changes in the E.coli concentration in a range from 10 to 102 CFU/mL.

Original languageEnglish
Title of host publication2021 IEEE International Workshop on Metrology for AeroSpace, MetroAeroSpace 2021 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages469-472
Number of pages4
ISBN (Electronic)9781728175560
DOIs
StatePublished - 2021.06.23
Event8th IEEE International Workshop on Metrology for AeroSpace, MetroAeroSpace 2021 - Virtual, Online
Duration: 2021.06.222021.06.25

Publication series

Name2021 IEEE International Workshop on Metrology for AeroSpace, MetroAeroSpace 2021 - Proceedings

Conference

Conference8th IEEE International Workshop on Metrology for AeroSpace, MetroAeroSpace 2021
CityVirtual, Online
Period21.06.2221.06.25

Keywords

  • Bacteria
  • Liquid crystals
  • Nanomaterials
  • Plasmonics
  • Water

Quacquarelli Symonds(QS) Subject Topics

  • Engineering - Mechanical
  • Engineering - Civil & Structural
  • Physics & Astronomy

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