Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

Shanta Raj Bhattarai; Ho Keun Yi; Narayan Bhattarai; Pyoung Han Hwang; Hak Yong Kim

doi:10.1016/j.actbio.2005.10.007

Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

Shanta Raj Bhattarai
, Ho Keun Yi
, Narayan Bhattarai
, Pyoung Han Hwang
, Hak Yong Kim^*

^*Corresponding author for this work

Department of Dentistry

Research output: Contribution to journal › Journal article › peer-review

19 Scopus citations

Abstract

The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.

Original language	English
Pages (from-to)	207-212
Number of pages	6
Journal	Acta Biomaterialia
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.actbio.2005.10.007
State	Published - 2006.03

Keywords

Biodegradable DNA
Cell proliferation
Cell transfection
Poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol)

Quacquarelli Symonds(QS) Subject Topics

Materials Science
Biological Sciences

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10.1016/j.actbio.2005.10.007

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title = "Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection",

abstract = "The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.",

keywords = "Biodegradable DNA, Cell proliferation, Cell transfection, Poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol)",

author = "Bhattarai, \{Shanta Raj\} and Yi, \{Ho Keun\} and Narayan Bhattarai and Hwang, \{Pyoung Han\} and Kim, \{Hak Yong\}",

year = "2006",

month = mar,

doi = "10.1016/j.actbio.2005.10.007",

language = "English",

volume = "2",

pages = "207--212",

journal = "Acta Biomaterialia",

issn = "1742-7061",

number = "2",

}

TY - JOUR

T1 - Novel block copolymer (PPDO/PLLA-b-PEG)

T2 - Enhancement of DNA uptake and cell transfection

AU - Bhattarai, Shanta Raj

AU - Yi, Ho Keun

AU - Bhattarai, Narayan

AU - Hwang, Pyoung Han

AU - Kim, Hak Yong

PY - 2006/3

Y1 - 2006/3

N2 - The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.

AB - The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.

KW - Biodegradable DNA

KW - Cell proliferation

KW - Cell transfection

KW - Poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol)

UR - https://www.scopus.com/pages/publications/32544449331

U2 - 10.1016/j.actbio.2005.10.007

DO - 10.1016/j.actbio.2005.10.007

M3 - Journal article

C2 - 16701879

AN - SCOPUS:32544449331

SN - 1742-7061

VL - 2

SP - 207

EP - 212

JO - Acta Biomaterialia

JF - Acta Biomaterialia

IS - 2

ER -

Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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