Optimization of ultraviolet irradiate conditions for vitamin D2 synthesis in shitake mushrooms (Lentinula edodes) by using response surface methodology

Ya Zhang; Wei Jie Wu; Geun Seoup Song; Byung Yong Ahn

doi:10.3839/jabc.2015.006

Optimization of ultraviolet irradiate conditions for vitamin D₂ synthesis in shitake mushrooms (Lentinula edodes) by using response surface methodology

Ya Zhang
, Wei Jie Wu
, Geun Seoup Song
, Byung Yong Ahn^*

^*Corresponding author for this work

Department of Food Science and Technology

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

7 Scopus citations

Abstract

The determination of the optimum conditions for the conversion of ergosterol to vitamin D₂ in shitake mushrooms (Lentinula edodes) was studied using response surface methodology (RSM). The effects of the three main variables ambient temperature (20–40°C), exposure time (60-180 min), and irradiation intensity (0.6–1.8 W/m²) were investigated. According to the RSM ridge analysis, the optimum conditions were as follows: ambient temperature of 34.2°C, exposure time of 175.6 min, and irradiation intensity of 1.41 W/m². Under these optimum conditions, the maximum vitamin D₂ content of 117.93 µg/g in shitake mushrooms was obtained, which agreed fairly well with the predicted value of 122.60 µg/g.

Original language	English
Pages (from-to)	25-29
Number of pages	5
Journal	Journal of Applied Biological Chemistry
Volume	58
Issue number	1
DOIs	https://doi.org/10.3839/jabc.2015.006
State	Published - 2015.03.1

Keywords

Ergosterol
Response surface methodology
Shitake mushroom
Ultraviolet irradiate
Vitamin D

Quacquarelli Symonds(QS) Subject Topics

Engineering - Petroleum
Engineering - Chemical
Chemistry

Access to Document

10.3839/jabc.2015.006

Cite this

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title = "Optimization of ultraviolet irradiate conditions for vitamin D2 synthesis in shitake mushrooms (Lentinula edodes) by using response surface methodology",

abstract = "The determination of the optimum conditions for the conversion of ergosterol to vitamin D2 in shitake mushrooms (Lentinula edodes) was studied using response surface methodology (RSM). The effects of the three main variables ambient temperature (20–40°C), exposure time (60-180 min), and irradiation intensity (0.6–1.8 W/m2) were investigated. According to the RSM ridge analysis, the optimum conditions were as follows: ambient temperature of 34.2°C, exposure time of 175.6 min, and irradiation intensity of 1.41 W/m2. Under these optimum conditions, the maximum vitamin D2 content of 117.93 µg/g in shitake mushrooms was obtained, which agreed fairly well with the predicted value of 122.60 µg/g.",

keywords = "Ergosterol, Response surface methodology, Shitake mushroom, Ultraviolet irradiate, Vitamin D",

author = "Ya Zhang and Wu, \{Wei Jie\} and Song, \{Geun Seoup\} and Ahn, \{Byung Yong\}",

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year = "2015",

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doi = "10.3839/jabc.2015.006",

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T1 - Optimization of ultraviolet irradiate conditions for vitamin D2 synthesis in shitake mushrooms (Lentinula edodes) by using response surface methodology

AU - Zhang, Ya

AU - Wu, Wei Jie

AU - Song, Geun Seoup

AU - Ahn, Byung Yong

N1 - Publisher Copyright: © The Korean Society for Applied Biological Chemistry 2015.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - The determination of the optimum conditions for the conversion of ergosterol to vitamin D2 in shitake mushrooms (Lentinula edodes) was studied using response surface methodology (RSM). The effects of the three main variables ambient temperature (20–40°C), exposure time (60-180 min), and irradiation intensity (0.6–1.8 W/m2) were investigated. According to the RSM ridge analysis, the optimum conditions were as follows: ambient temperature of 34.2°C, exposure time of 175.6 min, and irradiation intensity of 1.41 W/m2. Under these optimum conditions, the maximum vitamin D2 content of 117.93 µg/g in shitake mushrooms was obtained, which agreed fairly well with the predicted value of 122.60 µg/g.

AB - The determination of the optimum conditions for the conversion of ergosterol to vitamin D2 in shitake mushrooms (Lentinula edodes) was studied using response surface methodology (RSM). The effects of the three main variables ambient temperature (20–40°C), exposure time (60-180 min), and irradiation intensity (0.6–1.8 W/m2) were investigated. According to the RSM ridge analysis, the optimum conditions were as follows: ambient temperature of 34.2°C, exposure time of 175.6 min, and irradiation intensity of 1.41 W/m2. Under these optimum conditions, the maximum vitamin D2 content of 117.93 µg/g in shitake mushrooms was obtained, which agreed fairly well with the predicted value of 122.60 µg/g.

KW - Ergosterol

KW - Response surface methodology

KW - Shitake mushroom

KW - Ultraviolet irradiate

KW - Vitamin D

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