Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles

Seok Hee Hyun; Hee Kwon Kim; Jong Mok Kim; David H. Thompson

doi:10.1021/ja104204z

Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C₂₀BAS bolalipid membrane vesicles

Seok Hee Hyun
, Hee Kwon Kim
, Jong Mok Kim
, David H. Thompson

Department of Medicine

Purdue University

Research output: Contribution to journal › Journal article › peer-review

3 Scopus citations

Abstract

Ni²⁺:NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his₆ gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.

Original language	English
Pages (from-to)	17053-17055
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	132
Issue number	48
DOIs	https://doi.org/10.1021/ja104204z
State	Published - 2010.12.8

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10.1021/ja104204z

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@article{40218e5e58184a76b06044ed45845a7e,

title = "Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles",

abstract = "Ni2+:NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.",

author = "Hyun, \{Seok Hee\} and Kim, \{Hee Kwon\} and Kim, \{Jong Mok\} and Thompson, \{David H.\}",

year = "2010",

month = dec,

day = "8",

doi = "10.1021/ja104204z",

language = "English",

volume = "132",

pages = "17053--17055",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

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T1 - Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles

AU - Hyun, Seok Hee

AU - Kim, Hee Kwon

AU - Kim, Jong Mok

AU - Thompson, David H.

PY - 2010/12/8

Y1 - 2010/12/8

N2 - Ni2+:NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.

AB - Ni2+:NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.

UR - https://www.scopus.com/pages/publications/78650085135

U2 - 10.1021/ja104204z

DO - 10.1021/ja104204z

M3 - Journal article

C2 - 20715817

AN - SCOPUS:78650085135

SN - 0002-7863

VL - 132

SP - 17053

EP - 17055

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 48

ER -

Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C₂₀BAS bolalipid membrane vesicles

Abstract

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