Osteogenic differentiation of rat adipose stem cells in demineralized bone particles sponges

Demineralized bone particle (DBP) is a well-studied biomaterial, composed of collagen, calcium and proteoglycan. It is widely used in tissue engineering and regenerative medicine, because it contains powerful bioactive molecules that facilitate new bone or cartilage growth. Herein, we fabricated 1, 2 and 3% sponges using natural biomaterial DBP and studied its efficiency in rat adipose stem cells (rASCs) as models. After rASCs were seeded on DBP sponges, we evaluated rASCs adhesion, proliferation, cell attachment, proliferation and osteogenesis using SEM, MTT, and bone differentiation expose using ALP assay, RT-PCR in vitro. Histological examinations were performed at 1,4 and 8 weeks after implantation DBP sponge to assess the effect of bone differentiation in vivo using Alizarin Red S, Von Kossa staining. We found that 1% DBP sponge can provide suitable environment for bone differentiation of rASCs.