Oxalic acid from lentinula edodes culture filtrate: Antimicrobial activity on phytopathogenic bacteria and qualitative and quantitative analyses

  • A. Min Kwak
  • , In Kyoung Lee
  • , Sang Yeop Lee
  • , Bong Sik Yun
  • , Hee Wan Kang*
  • *Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases.

Original languageEnglish
Pages (from-to)338-342
Number of pages5
JournalMycobiology
Volume44
Issue number4
DOIs
StatePublished - 2016

Keywords

  • Antibacterial activity
  • Oxalic acid
  • Qualitative and quantitative analyses

Quacquarelli Symonds(QS) Subject Topics

  • Medicine
  • Biological Sciences

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