Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis

Shalem Raj Padakandla; Gun Woong Lee; Jong Chan Chae

doi:10.1007/s10482-015-0574-4

Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis

Shalem Raj Padakandla
, Gun Woong Lee
, Jong Chan Chae^*

^*Corresponding author for this work

Bioresource and Biomolecule Engineering

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

10 Scopus citations

Abstract

A rod shaped, Gram-stain positive, non-motile, facultative anaerobic and gelatin hydrolysing bacterium, strain PG1^T, was isolated from reclaimed land soil in Kyehwa-do, Republic of Korea. Strain PG1^T showed highest 16S rRNA gene sequence similarity (97.4 and 96.5 %, respectively) to Paenibacillus shenyangensis A9^T and Paenibacillus hunanensis FeL05^T, and clustered closely with the members of the family Paenibacillaceae. DNA–DNA hybridization studies revealed a genomic relatedness of 47 ± 9 % with P. shenyangensis A9^T. The predominant fatty acids of strain PG1^T were identified to be anteiso-C_15:0 (46.7 %) and C_16:0 (22.7 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were found to be the major polar lipids. The genomic DNA G+C content was found to be 47.7 mol%. This polyphasic characterisation of the newly isolated strain PG1^T justifies its description as representative of a novel species in the genus Paenibacillus, for which the name Paenibacillus gelatinilyticus sp. nov., (type strain = PG1^T = KCTC 33642^T = JCM 30624^T) is proposed.

Original language	English
Pages (from-to)	1197-1203
Number of pages	7
Journal	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume	108
Issue number	5
DOIs	https://doi.org/10.1007/s10482-015-0574-4
State	Published - 2015.11.1

Keywords

Paenibacillus gelatinilyticus
Polyphasic taxonomy
Reclaimed soil

Quacquarelli Symonds(QS) Subject Topics

Biological Sciences

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10.1007/s10482-015-0574-4

Cite this

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title = " Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis",

abstract = "A rod shaped, Gram-stain positive, non-motile, facultative anaerobic and gelatin hydrolysing bacterium, strain PG1T, was isolated from reclaimed land soil in Kyehwa-do, Republic of Korea. Strain PG1T showed highest 16S rRNA gene sequence similarity (97.4 and 96.5 \%, respectively) to Paenibacillus shenyangensis A9T and Paenibacillus hunanensis FeL05T, and clustered closely with the members of the family Paenibacillaceae. DNA–DNA hybridization studies revealed a genomic relatedness of 47 ± 9 \% with P. shenyangensis A9T. The predominant fatty acids of strain PG1T were identified to be anteiso-C15:0 (46.7 \%) and C16:0 (22.7 \%). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were found to be the major polar lipids. The genomic DNA G+C content was found to be 47.7 mol\%. This polyphasic characterisation of the newly isolated strain PG1T justifies its description as representative of a novel species in the genus Paenibacillus, for which the name Paenibacillus gelatinilyticus sp. nov., (type strain = PG1T = KCTC 33642T = JCM 30624T) is proposed.",

keywords = "Paenibacillus gelatinilyticus, Polyphasic taxonomy, Reclaimed soil",

author = "Padakandla, \{Shalem Raj\} and Lee, \{Gun Woong\} and Chae, \{Jong Chan\}",

note = "Publisher Copyright: {\textcopyright} 2015, Springer International Publishing Switzerland.",

year = "2015",

month = nov,

day = "1",

doi = "10.1007/s10482-015-0574-4",

language = "English",

volume = "108",

pages = "1197--1203",

journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",

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Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis. / Padakandla, Shalem Raj; Lee, Gun Woong; Chae, Jong Chan.
In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, Vol. 108, No. 5, 01.11.2015, p. 1197-1203.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis

AU - Padakandla, Shalem Raj

AU - Lee, Gun Woong

AU - Chae, Jong Chan

PY - 2015/11/1

Y1 - 2015/11/1

N2 - A rod shaped, Gram-stain positive, non-motile, facultative anaerobic and gelatin hydrolysing bacterium, strain PG1T, was isolated from reclaimed land soil in Kyehwa-do, Republic of Korea. Strain PG1T showed highest 16S rRNA gene sequence similarity (97.4 and 96.5 %, respectively) to Paenibacillus shenyangensis A9T and Paenibacillus hunanensis FeL05T, and clustered closely with the members of the family Paenibacillaceae. DNA–DNA hybridization studies revealed a genomic relatedness of 47 ± 9 % with P. shenyangensis A9T. The predominant fatty acids of strain PG1T were identified to be anteiso-C15:0 (46.7 %) and C16:0 (22.7 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were found to be the major polar lipids. The genomic DNA G+C content was found to be 47.7 mol%. This polyphasic characterisation of the newly isolated strain PG1T justifies its description as representative of a novel species in the genus Paenibacillus, for which the name Paenibacillus gelatinilyticus sp. nov., (type strain = PG1T = KCTC 33642T = JCM 30624T) is proposed.

AB - A rod shaped, Gram-stain positive, non-motile, facultative anaerobic and gelatin hydrolysing bacterium, strain PG1T, was isolated from reclaimed land soil in Kyehwa-do, Republic of Korea. Strain PG1T showed highest 16S rRNA gene sequence similarity (97.4 and 96.5 %, respectively) to Paenibacillus shenyangensis A9T and Paenibacillus hunanensis FeL05T, and clustered closely with the members of the family Paenibacillaceae. DNA–DNA hybridization studies revealed a genomic relatedness of 47 ± 9 % with P. shenyangensis A9T. The predominant fatty acids of strain PG1T were identified to be anteiso-C15:0 (46.7 %) and C16:0 (22.7 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were found to be the major polar lipids. The genomic DNA G+C content was found to be 47.7 mol%. This polyphasic characterisation of the newly isolated strain PG1T justifies its description as representative of a novel species in the genus Paenibacillus, for which the name Paenibacillus gelatinilyticus sp. nov., (type strain = PG1T = KCTC 33642T = JCM 30624T) is proposed.

KW - Paenibacillus gelatinilyticus

KW - Polyphasic taxonomy

KW - Reclaimed soil

UR - https://www.scopus.com/pages/publications/84943815967

U2 - 10.1007/s10482-015-0574-4

DO - 10.1007/s10482-015-0574-4

M3 - Journal article

C2 - 26319400

AN - SCOPUS:84943815967

SN - 0003-6072

VL - 108

SP - 1197

EP - 1203

JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

IS - 5

ER -

Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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