Peptidases affecting recombinant protein production by Streptomyces lividans

Soon II Yun; Ahmad R.M. Yahya; Marco Malten; Doug Cossar; William A. Anderson; Jeno M. Scharer; Murray Moo-Young

doi:10.1139/cjm-47-12-1137

Peptidases affecting recombinant protein production by Streptomyces lividans

Soon II Yun
, Ahmad R.M. Yahya
, Marco Malten
, Doug Cossar
, William A. Anderson^*
, Jeno M. Scharer
, Murray Moo-Young

^*Corresponding author for this work

Department of Food Science and Technology

University of Waterloo

Research output: Contribution to journal › Journal article › peer-review

8 Scopus citations

Abstract

The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.

Original language	English
Pages (from-to)	1137-1140
Number of pages	4
Journal	Canadian Journal of Microbiology
Volume	47
Issue number	12
DOIs	https://doi.org/10.1139/cjm-47-12-1137
State	Published - 2001

Keywords

Fermentation
Interleukin-3
Proteases
Streptomyces
Tripeptidyl peptidase

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10.1139/cjm-47-12-1137

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title = "Peptidases affecting recombinant protein production by Streptomyces lividans",

abstract = "The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.",

keywords = "Fermentation, Interleukin-3, Proteases, Streptomyces, Tripeptidyl peptidase",

author = "Yun, \{Soon II\} and Yahya, \{Ahmad R.M.\} and Marco Malten and Doug Cossar and Anderson, \{William A.\} and Scharer, \{Jeno M.\} and Murray Moo-Young",

year = "2001",

doi = "10.1139/cjm-47-12-1137",

language = "English",

volume = "47",

pages = "1137--1140",

journal = "Canadian Journal of Microbiology",

issn = "0008-4166",

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TY - JOUR

T1 - Peptidases affecting recombinant protein production by Streptomyces lividans

AU - Yun, Soon II

AU - Yahya, Ahmad R.M.

AU - Malten, Marco

AU - Cossar, Doug

AU - Anderson, William A.

AU - Scharer, Jeno M.

AU - Moo-Young, Murray

PY - 2001

Y1 - 2001

N2 - The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.

AB - The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.

KW - Fermentation

KW - Interleukin-3

KW - Proteases

KW - Streptomyces

KW - Tripeptidyl peptidase

UR - https://www.scopus.com/pages/publications/0035722969

U2 - 10.1139/cjm-47-12-1137

DO - 10.1139/cjm-47-12-1137

M3 - Journal article

C2 - 11822841

AN - SCOPUS:0035722969

SN - 0008-4166

VL - 47

SP - 1137

EP - 1140

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

IS - 12

ER -

Peptidases affecting recombinant protein production by Streptomyces lividans

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Keywords

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