Phylogenetic analysis and characterization of Korean orf virus from dairy goats: Case report

Jae Ku Oem; In Soon Roh; Kyung Hyun Lee; Kyoung Ki Lee; Hye Ryoung Kim; Young Hwa Jean; O. Soo Lee

doi:10.1186/1743-422X-6-167

Phylogenetic analysis and characterization of Korean orf virus from dairy goats: Case report

Jae Ku Oem^*
, In Soon Roh
, Kyung Hyun Lee
, Kyoung Ki Lee
, Hye Ryoung Kim
, Young Hwa Jean
, O. Soo Lee

^*Corresponding author for this work

Department of Veterinary Medicine

Animal and Plant Quarantine Agency

Research output: Contribution to journal › Journal article › peer-review

43 Scopus citations

Abstract

An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain (ORF/09/Korea), the sequences of the major envelope protein (B2L) and orf virus interferon resistance (VIR) genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF/09/Korea strain was closest to the isolates (Taiping) from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea.

Original language	English
Article number	167
Journal	Virology Journal
Volume	6
DOIs	https://doi.org/10.1186/1743-422X-6-167
State	Published - 2009

Access to Document

10.1186/1743-422X-6-167

Cite this

@article{7e976dd02f6b4174970e8a25c841a9b1,

title = "Phylogenetic analysis and characterization of Korean orf virus from dairy goats: Case report",

abstract = "An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain (ORF/09/Korea), the sequences of the major envelope protein (B2L) and orf virus interferon resistance (VIR) genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF/09/Korea strain was closest to the isolates (Taiping) from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea.",

author = "Oem, \{Jae Ku\} and Roh, \{In Soon\} and Lee, \{Kyung Hyun\} and Lee, \{Kyoung Ki\} and Kim, \{Hye Ryoung\} and Jean, \{Young Hwa\} and Lee, \{O. Soo\}",

year = "2009",

doi = "10.1186/1743-422X-6-167",

language = "English",

volume = "6",

journal = "Virology Journal",

issn = "1743-422X",

}

TY - JOUR

T1 - Phylogenetic analysis and characterization of Korean orf virus from dairy goats

T2 - Case report

AU - Oem, Jae Ku

AU - Roh, In Soon

AU - Lee, Kyung Hyun

AU - Lee, Kyoung Ki

AU - Kim, Hye Ryoung

AU - Jean, Young Hwa

AU - Lee, O. Soo

PY - 2009

Y1 - 2009

N2 - An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain (ORF/09/Korea), the sequences of the major envelope protein (B2L) and orf virus interferon resistance (VIR) genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF/09/Korea strain was closest to the isolates (Taiping) from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea.

AB - An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain (ORF/09/Korea), the sequences of the major envelope protein (B2L) and orf virus interferon resistance (VIR) genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF/09/Korea strain was closest to the isolates (Taiping) from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea.

UR - https://www.scopus.com/pages/publications/70450228613

U2 - 10.1186/1743-422X-6-167

DO - 10.1186/1743-422X-6-167

M3 - Journal article

C2 - 19835585

AN - SCOPUS:70450228613

SN - 1743-422X

VL - 6

JO - Virology Journal

JF - Virology Journal

M1 - 167

ER -

Phylogenetic analysis and characterization of Korean orf virus from dairy goats: Case report

Abstract

Access to Document

Other files and links

Fingerprint

Cite this