Pooling upper respiratory specimens for rapid mass screening of COVID-19 by real-time RT-PCR

So Yeon Kim; Jaehyeon Lee; Heungsup Sung; Hyukmin Lee; Myung Guk Han; Cheon Kwon Yoo; Sang Won Lee; Ki Ho Hong

doi:10.3201/eid2610.201955

Pooling upper respiratory specimens for rapid mass screening of COVID-19 by real-time RT-PCR

So Yeon Kim
, Jaehyeon Lee
, Heungsup Sung
, Hyukmin Lee
, Myung Guk Han
, Cheon Kwon Yoo
, Sang Won Lee
, Ki Ho Hong^*

^*Corresponding author for this work

Department of Medicine

Research output: Contribution to journal › Journal article › peer-review

30 Scopus citations

Abstract

To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.

Original language	English
Pages (from-to)	2469-2472
Number of pages	4
Journal	Emerging Infectious Diseases
Volume	26
Issue number	10
DOIs	https://doi.org/10.3201/eid2610.201955
State	Published - 2020.10

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SDG 3 Good Health and Well-being

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10.3201/eid2610.201955

Cite this

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title = "Pooling upper respiratory specimens for rapid mass screening of COVID-19 by real-time RT-PCR",

abstract = "To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.",

author = "Kim, \{So Yeon\} and Jaehyeon Lee and Heungsup Sung and Hyukmin Lee and Han, \{Myung Guk\} and Yoo, \{Cheon Kwon\} and Lee, \{Sang Won\} and Hong, \{Ki Ho\}",

year = "2020",

month = oct,

doi = "10.3201/eid2610.201955",

language = "English",

volume = "26",

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AU - Kim, So Yeon

AU - Lee, Jaehyeon

AU - Sung, Heungsup

AU - Lee, Hyukmin

AU - Han, Myung Guk

AU - Yoo, Cheon Kwon

AU - Lee, Sang Won

AU - Hong, Ki Ho

PY - 2020/10

Y1 - 2020/10

N2 - To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.

AB - To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.

UR - https://www.scopus.com/pages/publications/85091263934

U2 - 10.3201/eid2610.201955

DO - 10.3201/eid2610.201955

M3 - Journal article

C2 - 32844739

AN - SCOPUS:85091263934

SN - 1080-6040

VL - 26

SP - 2469

EP - 2472

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 10

ER -

Pooling upper respiratory specimens for rapid mass screening of COVID-19 by real-time RT-PCR

Abstract

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