Preparation and BDNF release profile of BDNF-loaded PLGA scaffolds for tissue engineered nerve regeneration

Chomin Kim; Hee Kim Soon; Young Oh A; Ah Kim Geun; Woo Lee Ii; John M. Rhee; Gilson Khang

Preparation and BDNF release profile of BDNF-loaded PLGA scaffolds for tissue engineered nerve regeneration

Chomin Kim
, Hee Kim Soon
, Young Oh A
, Ah Kim Geun
, Woo Lee Ii
, John M. Rhee
, Gilson Khang^*

^*Corresponding author for this work

Department of Polymer -Nano Science &Technology

Jeonbuk National University
The Catholic University of Korea

Research output: Contribution to journal › Journal article › peer-review

2 Scopus citations

Abstract

We manufactured poly(L-lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle(DBP) and hyaluronicacid(HA) by ice-particle leaching method and tested their ability of sustained release of brain derived neurotrophic factor(BDNF). BDNF(50 and 200 ng) mixed with PLGA, DBP/PLGA, HA/PLGA and DBP/HA/PLGA scaffold. The release profiles of BDNF from BDNF loaded scaffolds were assayed using ELISA. Morphological changes of scaffolds by BDNF release were also observed by SEM. BDNF stably and sustainedly released from DBP/HA/PLGA than from PLGA and DBP/PLGA scaffolds. DBP/HA/PLGA scaffolds showed the great structural changes, which demonstrated BDNF release amount from DBP/HA/PLGA scaffolds were highest in all groups. We suggest that BDNF loaded DBP/HA/PLGA scaffold would be very useful for nerve regeneration.

Original language	English
Pages (from-to)	529-536
Number of pages	8
Journal	Polymer (Korea)
Volume	32
Issue number	6
State	Published - 2008.11

Keywords

Brain derived neurotrophic factor
Demineralized bone particle
Hyaluronic acid
Poly(L-lactide-co-glycolide)
Scaffold
Spinal cord regeneration

Quacquarelli Symonds(QS) Subject Topics

Materials Science
Engineering - Chemical

Cite this

@article{0c32c9eecb494ee2b2a1b8533dcfdedd,

title = "Preparation and BDNF release profile of BDNF-loaded PLGA scaffolds for tissue engineered nerve regeneration",

abstract = "We manufactured poly(L-lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle(DBP) and hyaluronicacid(HA) by ice-particle leaching method and tested their ability of sustained release of brain derived neurotrophic factor(BDNF). BDNF(50 and 200 ng) mixed with PLGA, DBP/PLGA, HA/PLGA and DBP/HA/PLGA scaffold. The release profiles of BDNF from BDNF loaded scaffolds were assayed using ELISA. Morphological changes of scaffolds by BDNF release were also observed by SEM. BDNF stably and sustainedly released from DBP/HA/PLGA than from PLGA and DBP/PLGA scaffolds. DBP/HA/PLGA scaffolds showed the great structural changes, which demonstrated BDNF release amount from DBP/HA/PLGA scaffolds were highest in all groups. We suggest that BDNF loaded DBP/HA/PLGA scaffold would be very useful for nerve regeneration.",

keywords = "Brain derived neurotrophic factor, Demineralized bone particle, Hyaluronic acid, Poly(L-lactide-co-glycolide), Scaffold, Spinal cord regeneration",

author = "Chomin Kim and Soon, \{Hee Kim\} and A, \{Young Oh\} and Geun, \{Ah Kim\} and Ii, \{Woo Lee\} and Rhee, \{John M.\} and Gilson Khang",

year = "2008",

month = nov,

language = "English",

volume = "32",

pages = "529--536",

journal = "Polymer (Korea)",

issn = "0379-153X",

number = "6",

}

TY - JOUR

T1 - Preparation and BDNF release profile of BDNF-loaded PLGA scaffolds for tissue engineered nerve regeneration

AU - Kim, Chomin

AU - Soon, Hee Kim

AU - A, Young Oh

AU - Geun, Ah Kim

AU - Ii, Woo Lee

AU - Rhee, John M.

AU - Khang, Gilson

PY - 2008/11

Y1 - 2008/11

N2 - We manufactured poly(L-lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle(DBP) and hyaluronicacid(HA) by ice-particle leaching method and tested their ability of sustained release of brain derived neurotrophic factor(BDNF). BDNF(50 and 200 ng) mixed with PLGA, DBP/PLGA, HA/PLGA and DBP/HA/PLGA scaffold. The release profiles of BDNF from BDNF loaded scaffolds were assayed using ELISA. Morphological changes of scaffolds by BDNF release were also observed by SEM. BDNF stably and sustainedly released from DBP/HA/PLGA than from PLGA and DBP/PLGA scaffolds. DBP/HA/PLGA scaffolds showed the great structural changes, which demonstrated BDNF release amount from DBP/HA/PLGA scaffolds were highest in all groups. We suggest that BDNF loaded DBP/HA/PLGA scaffold would be very useful for nerve regeneration.

AB - We manufactured poly(L-lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle(DBP) and hyaluronicacid(HA) by ice-particle leaching method and tested their ability of sustained release of brain derived neurotrophic factor(BDNF). BDNF(50 and 200 ng) mixed with PLGA, DBP/PLGA, HA/PLGA and DBP/HA/PLGA scaffold. The release profiles of BDNF from BDNF loaded scaffolds were assayed using ELISA. Morphological changes of scaffolds by BDNF release were also observed by SEM. BDNF stably and sustainedly released from DBP/HA/PLGA than from PLGA and DBP/PLGA scaffolds. DBP/HA/PLGA scaffolds showed the great structural changes, which demonstrated BDNF release amount from DBP/HA/PLGA scaffolds were highest in all groups. We suggest that BDNF loaded DBP/HA/PLGA scaffold would be very useful for nerve regeneration.

KW - Brain derived neurotrophic factor

KW - Demineralized bone particle

KW - Hyaluronic acid

KW - Poly(L-lactide-co-glycolide)

KW - Scaffold

KW - Spinal cord regeneration

UR - https://www.scopus.com/pages/publications/57649105273

M3 - Journal article

AN - SCOPUS:57649105273

SN - 0379-153X

VL - 32

SP - 529

EP - 536

JO - Polymer (Korea)

JF - Polymer (Korea)

IS - 6

ER -

Preparation and BDNF release profile of BDNF-loaded PLGA scaffolds for tissue engineered nerve regeneration

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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