Presence of cu-type (Nirk) and cd1-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.

Jeonghwan Jang; Naoaki Ashida; Ayaaki Kai; Kazuo Isobe; Tomoyasu Nishizawa; Shigeto Otsuka; Akira Yokota; Keishi Senoo; Satoshi Ishii

doi:10.1264/jsme2.ME18039

Presence of cu-type (Nirk) and cd₁-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.

Jeonghwan Jang
, Naoaki Ashida
, Ayaaki Kai
, Kazuo Isobe
, Tomoyasu Nishizawa
, Shigeto Otsuka
, Akira Yokota
, Keishi Senoo
, Satoshi Ishii^*

^*Corresponding author for this work

Bioresource and Biomolecule Engineering

University of Minnesota Twin Cities
The University of Tokyo
Ibaraki University
Tohoku University

Research output: Contribution to journal › Journal article › peer-review

40 Scopus citations

Abstract

Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd₁-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1^T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1^T (=JCM 18858^T=KCTC 62391^T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

Original language	English
Pages (from-to)	326-331
Number of pages	6
Journal	Microbes and Environments
Volume	33
Issue number	3
DOIs	https://doi.org/10.1264/jsme2.ME18039
State	Published - 2018

Keywords

Bradyrhizobium
Denitrification
Genome
New species

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10.1264/jsme2.ME18039

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title = "Presence of cu-type (Nirk) and cd1-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.",

abstract = "Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd1-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1T (=JCM 18858T=KCTC 62391T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.",

keywords = "Bradyrhizobium, Denitrification, Genome, New species",

author = "Jeonghwan Jang and Naoaki Ashida and Ayaaki Kai and Kazuo Isobe and Tomoyasu Nishizawa and Shigeto Otsuka and Akira Yokota and Keishi Senoo and Satoshi Ishii",

year = "2018",

doi = "10.1264/jsme2.ME18039",

language = "English",

volume = "33",

pages = "326--331",

journal = "Microbes and Environments",

issn = "1342-6311",

number = "3",

}

TY - JOUR

T1 - Presence of cu-type (Nirk) and cd1-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.

AU - Jang, Jeonghwan

AU - Ashida, Naoaki

AU - Kai, Ayaaki

AU - Isobe, Kazuo

AU - Nishizawa, Tomoyasu

AU - Otsuka, Shigeto

AU - Yokota, Akira

AU - Senoo, Keishi

AU - Ishii, Satoshi

PY - 2018

Y1 - 2018

N2 - Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd1-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1T (=JCM 18858T=KCTC 62391T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

AB - Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd1-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1T (=JCM 18858T=KCTC 62391T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

KW - Bradyrhizobium

KW - Denitrification

KW - Genome

KW - New species

UR - https://www.scopus.com/pages/publications/85054395592

U2 - 10.1264/jsme2.ME18039

DO - 10.1264/jsme2.ME18039

M3 - Journal article

C2 - 30158366

AN - SCOPUS:85054395592

SN - 1342-6311

VL - 33

SP - 326

EP - 331

JO - Microbes and Environments

JF - Microbes and Environments

IS - 3

ER -

Presence of cu-type (Nirk) and cd₁-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.

Abstract

Keywords

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