Probe development for detection of TERRA 1 intramolecular G-quadruplex formation using a fluorescent adenosine derivative

In Sun Kim; Young Jun Seo

doi:10.1016/j.bmcl.2014.01.068

Probe development for detection of TERRA 1 intramolecular G-quadruplex formation using a fluorescent adenosine derivative

In Sun Kim
, Young Jun Seo^*

^*Corresponding author for this work

Department of Chemistry

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

21 Scopus citations

Abstract

We developed a probing system to detect the intramolecular G-quadruplex of telomeric repeat-containing RNA (TERRA 1). We used a fluorescent adenosine derivative rA^py as a fluorophore and incorporated it into the dangling position of the parallel-type G-quadruplex sequence of TERRA 1. The rA^py-modified G-quadruplex structure exhibited a strong fluorescence emission signal, while the emission signals of the single-strand and duplex structures were much lower.

Original language	English
Pages (from-to)	1589-1591
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	24
Issue number	6
DOIs	https://doi.org/10.1016/j.bmcl.2014.01.068
State	Published - 2014.03.15

Keywords

Fluorophore
G-quadruplex
Probe
RNA
TERRA 1

Quacquarelli Symonds(QS) Subject Topics

Medicine
Engineering - Petroleum
Pharmacy & Pharmacology
Chemistry
Biological Sciences

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10.1016/j.bmcl.2014.01.068

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title = "Probe development for detection of TERRA 1 intramolecular G-quadruplex formation using a fluorescent adenosine derivative",

abstract = "We developed a probing system to detect the intramolecular G-quadruplex of telomeric repeat-containing RNA (TERRA 1). We used a fluorescent adenosine derivative rApy as a fluorophore and incorporated it into the dangling position of the parallel-type G-quadruplex sequence of TERRA 1. The rApy-modified G-quadruplex structure exhibited a strong fluorescence emission signal, while the emission signals of the single-strand and duplex structures were much lower.",

keywords = "Fluorophore, G-quadruplex, Probe, RNA, TERRA 1",

author = "Kim, \{In Sun\} and Seo, \{Young Jun\}",

year = "2014",

month = mar,

day = "15",

doi = "10.1016/j.bmcl.2014.01.068",

language = "English",

volume = "24",

pages = "1589--1591",

journal = "Bioorganic and Medicinal Chemistry Letters",

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T1 - Probe development for detection of TERRA 1 intramolecular G-quadruplex formation using a fluorescent adenosine derivative

AU - Kim, In Sun

AU - Seo, Young Jun

PY - 2014/3/15

Y1 - 2014/3/15

N2 - We developed a probing system to detect the intramolecular G-quadruplex of telomeric repeat-containing RNA (TERRA 1). We used a fluorescent adenosine derivative rApy as a fluorophore and incorporated it into the dangling position of the parallel-type G-quadruplex sequence of TERRA 1. The rApy-modified G-quadruplex structure exhibited a strong fluorescence emission signal, while the emission signals of the single-strand and duplex structures were much lower.

AB - We developed a probing system to detect the intramolecular G-quadruplex of telomeric repeat-containing RNA (TERRA 1). We used a fluorescent adenosine derivative rApy as a fluorophore and incorporated it into the dangling position of the parallel-type G-quadruplex sequence of TERRA 1. The rApy-modified G-quadruplex structure exhibited a strong fluorescence emission signal, while the emission signals of the single-strand and duplex structures were much lower.

KW - Fluorophore

KW - G-quadruplex

KW - Probe

KW - RNA

KW - TERRA 1

UR - https://www.scopus.com/pages/publications/84895815659

U2 - 10.1016/j.bmcl.2014.01.068

DO - 10.1016/j.bmcl.2014.01.068

M3 - Journal article

C2 - 24513043

AN - SCOPUS:84895815659

SN - 0960-894X

VL - 24

SP - 1589

EP - 1591

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 6

ER -

Probe development for detection of TERRA 1 intramolecular G-quadruplex formation using a fluorescent adenosine derivative

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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