Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

Kandasamy Selvam; Muthusamy Govarthanan; Seralathan Kamala-Kannan; Munisamy Govindharaju; Balakrishnan Senthilkumar; Thangasamy Selvankumar; Arumugam Sengottaiyan

doi:10.1039/c4ra00066h

Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

Kandasamy Selvam
, Muthusamy Govarthanan
, Seralathan Kamala-Kannan
, Munisamy Govindharaju
, Balakrishnan Senthilkumar^*
, Thangasamy Selvankumar
, Arumugam Sengottaiyan

^*Corresponding author for this work

Environmental Biotechnology

Research output: Contribution to journal › Journal article › peer-review

43 Scopus citations

Abstract

The aim of this study was to assess the mixed combination of coffee pulp waste (CPW) and pineapple waste (PW) residues for cellulase production using newly isolated Acinetobacter sp. TSK-MASC in solid state fermentation. Response surface methodology based Box-Behnken design (BBD) was employed to optimize variables such as pH, incubation time, concentrations of CPW and PW. The BBD design investigation showed a sound adjustment of the quadratic model with the experimental statistics. Statistics based 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and culture parameters. The higher production (888 U mL ^-1) was achieved after 60 h of incubation with 3.0 g L^-1 of CPW and PW at pH 7.0. This journal is

Original language	English
Pages (from-to)	13045-13051
Number of pages	7
Journal	RSC Advances
Volume	4
Issue number	25
DOIs	https://doi.org/10.1039/c4ra00066h
State	Published - 2014

Quacquarelli Symonds(QS) Subject Topics

Engineering - Chemical
Chemistry

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10.1039/c4ra00066h

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title = "Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC",

abstract = "The aim of this study was to assess the mixed combination of coffee pulp waste (CPW) and pineapple waste (PW) residues for cellulase production using newly isolated Acinetobacter sp. TSK-MASC in solid state fermentation. Response surface methodology based Box-Behnken design (BBD) was employed to optimize variables such as pH, incubation time, concentrations of CPW and PW. The BBD design investigation showed a sound adjustment of the quadratic model with the experimental statistics. Statistics based 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and culture parameters. The higher production (888 U mL -1) was achieved after 60 h of incubation with 3.0 g L-1 of CPW and PW at pH 7.0. This journal is",

author = "Kandasamy Selvam and Muthusamy Govarthanan and Seralathan Kamala-Kannan and Munisamy Govindharaju and Balakrishnan Senthilkumar and Thangasamy Selvankumar and Arumugam Sengottaiyan",

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doi = "10.1039/c4ra00066h",

language = "English",

volume = "4",

pages = "13045--13051",

journal = "RSC Advances",

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T1 - Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

AU - Selvam, Kandasamy

AU - Govarthanan, Muthusamy

AU - Kamala-Kannan, Seralathan

AU - Govindharaju, Munisamy

AU - Senthilkumar, Balakrishnan

AU - Selvankumar, Thangasamy

AU - Sengottaiyan, Arumugam

PY - 2014

Y1 - 2014

N2 - The aim of this study was to assess the mixed combination of coffee pulp waste (CPW) and pineapple waste (PW) residues for cellulase production using newly isolated Acinetobacter sp. TSK-MASC in solid state fermentation. Response surface methodology based Box-Behnken design (BBD) was employed to optimize variables such as pH, incubation time, concentrations of CPW and PW. The BBD design investigation showed a sound adjustment of the quadratic model with the experimental statistics. Statistics based 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and culture parameters. The higher production (888 U mL -1) was achieved after 60 h of incubation with 3.0 g L-1 of CPW and PW at pH 7.0. This journal is

AB - The aim of this study was to assess the mixed combination of coffee pulp waste (CPW) and pineapple waste (PW) residues for cellulase production using newly isolated Acinetobacter sp. TSK-MASC in solid state fermentation. Response surface methodology based Box-Behnken design (BBD) was employed to optimize variables such as pH, incubation time, concentrations of CPW and PW. The BBD design investigation showed a sound adjustment of the quadratic model with the experimental statistics. Statistics based 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and culture parameters. The higher production (888 U mL -1) was achieved after 60 h of incubation with 3.0 g L-1 of CPW and PW at pH 7.0. This journal is

UR - https://www.scopus.com/pages/publications/84896742456

U2 - 10.1039/c4ra00066h

DO - 10.1039/c4ra00066h

M3 - Journal article

AN - SCOPUS:84896742456

SN - 2046-2069

VL - 4

SP - 13045

EP - 13051

JO - RSC Advances

JF - RSC Advances

IS - 25

ER -

Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

Abstract

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