Abstract
Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, {increment}ldhA/pBR-iBO (ilvIH-ilvC-ilvD-kivd-adhA), produced 2-butanol (160 mg l-1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l-1. This represents the first successful attempt to produce 2-butanol from crude glycerol.
| Original language | English |
|---|---|
| Pages (from-to) | 57-62 |
| Number of pages | 6 |
| Journal | Biotechnology Letters |
| Volume | 36 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2014.01 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- 2-Butanol/isobutanol
- Crude glycerol
- Genetic engineering
- Klebsiella pneumoniae
Quacquarelli Symonds(QS) Subject Topics
- Engineering - Chemical
- Biological Sciences
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