Protective effects of edible insect protein extracts from Protaetia brevitarsis against H2O2-induced oxidative stress in mouse C2C12 myoblast cells

Jae Hoon Lee; Tae Kyung Kim; Min Cheol Kang; Bum Keun Kim; Yun Sang Choi

doi:10.1016/j.fbio.2023.102396

Protective effects of edible insect protein extracts from Protaetia brevitarsis against H₂O₂-induced oxidative stress in mouse C2C12 myoblast cells

Jae Hoon Lee
, Tae Kyung Kim
, Min Cheol Kang
, Bum Keun Kim
, Yun Sang Choi^*

^*Corresponding author for this work

Department of Food Science and Technology

Korea Food Research Institute

Research output: Contribution to journal › Journal article › peer-review

27 Scopus citations

Abstract

Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity. In this study, the apoptotic protective effects of water-soluble protein (WPB) and salt-soluble protein (SPB) extracts derived from Protaetia brevitarsis against hydrogen peroxide (H₂O₂)-induced oxidative stress in C2C12 cells were investigated. WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities. In addition, WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H₂O₂ treatment, and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro- and anti-apoptotic proteins. Therefore, our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.

Original language	English
Article number	102396
Journal	Food Bioscience
Volume	52
DOIs	https://doi.org/10.1016/j.fbio.2023.102396
State	Published - 2023.04

Keywords

Anti-Apoptosis
Antioxidant
C2C12 myoblast cells
Edible insect protein extract
Protaetia brevitarsis

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10.1016/j.fbio.2023.102396

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@article{59694abcb419447eb3f4f908813563ac,

title = "Protective effects of edible insect protein extracts from Protaetia brevitarsis against H2O2-induced oxidative stress in mouse C2C12 myoblast cells",

abstract = "Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity. In this study, the apoptotic protective effects of water-soluble protein (WPB) and salt-soluble protein (SPB) extracts derived from Protaetia brevitarsis against hydrogen peroxide (H2O2)-induced oxidative stress in C2C12 cells were investigated. WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities. In addition, WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment, and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro- and anti-apoptotic proteins. Therefore, our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.",

keywords = "Anti-Apoptosis, Antioxidant, C2C12 myoblast cells, Edible insect protein extract, Protaetia brevitarsis",

author = "Lee, \{Jae Hoon\} and Kim, \{Tae Kyung\} and Kang, \{Min Cheol\} and Kim, \{Bum Keun\} and Choi, \{Yun Sang\}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = apr,

doi = "10.1016/j.fbio.2023.102396",

language = "English",

volume = "52",

journal = "Food Bioscience",

issn = "2212-4292",

}

TY - JOUR

T1 - Protective effects of edible insect protein extracts from Protaetia brevitarsis against H2O2-induced oxidative stress in mouse C2C12 myoblast cells

AU - Lee, Jae Hoon

AU - Kim, Tae Kyung

AU - Kang, Min Cheol

AU - Kim, Bum Keun

AU - Choi, Yun Sang

PY - 2023/4

Y1 - 2023/4

N2 - Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity. In this study, the apoptotic protective effects of water-soluble protein (WPB) and salt-soluble protein (SPB) extracts derived from Protaetia brevitarsis against hydrogen peroxide (H2O2)-induced oxidative stress in C2C12 cells were investigated. WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities. In addition, WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment, and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro- and anti-apoptotic proteins. Therefore, our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.

AB - Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity. In this study, the apoptotic protective effects of water-soluble protein (WPB) and salt-soluble protein (SPB) extracts derived from Protaetia brevitarsis against hydrogen peroxide (H2O2)-induced oxidative stress in C2C12 cells were investigated. WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities. In addition, WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment, and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro- and anti-apoptotic proteins. Therefore, our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.

KW - Anti-Apoptosis

KW - Antioxidant

KW - C2C12 myoblast cells

KW - Edible insect protein extract

KW - Protaetia brevitarsis

UR - https://www.scopus.com/pages/publications/85146583345

U2 - 10.1016/j.fbio.2023.102396

DO - 10.1016/j.fbio.2023.102396

M3 - Journal article

AN - SCOPUS:85146583345

SN - 2212-4292

VL - 52

JO - Food Bioscience

JF - Food Bioscience

M1 - 102396

ER -

Protective effects of edible insect protein extracts from Protaetia brevitarsis against H₂O₂-induced oxidative stress in mouse C2C12 myoblast cells

Abstract

Keywords

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