Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin

Dong Jun Park; Simranjeet Singh Sekhon; Ji Young Ahn; Hobaek Yoon; Jung Ho Ko; Lyon Lee; Yang Hoon Kim; Jiho Min

doi:10.1007/s13530-016-0256-2

Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin

Dong Jun Park
, Simranjeet Singh Sekhon
, Ji Young Ahn
, Hobaek Yoon
, Jung Ho Ko
, Lyon Lee
, Yang Hoon Kim
, Jiho Min^*

^*Corresponding author for this work

Division of Chemical Engineering

Chungbuk National University
Rural Development Administration
Western University of Health Sciences

Research output: Contribution to journal › Journal article › peer-review

2 Scopus citations

Abstract

A high quantity of melanin causes hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes much melanin. In this study, lysosomal proteins were identified through two-dimensional polyacrylamide gel electrophoresis (2-DE) after melanocytes were exposed to melanin (B16F10). Melanocytes were exposed to 100 ppm melanin for 24 hours and the lysosomal proteins were extracted. The results showed that 18 protein spots up regulated and 6 protein spots down regulated after melanin exposure. The expression pattern of established lysosomal proteins is informative in understanding proteins related to decreasing melanin.

Original language	English
Pages (from-to)	7-11
Number of pages	5
Journal	Toxicology and Environmental Health Sciences
Volume	8
Issue number	1
DOIs	https://doi.org/10.1007/s13530-016-0256-2
State	Published - 2016.03.1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

B16F10
Lysosomal proteins
Melanin
Melanocyte
Two-dimensional electrophoresis (2-DE)

Quacquarelli Symonds(QS) Subject Topics

Environmental Sciences
Pharmacy & Pharmacology

Access to Document

10.1007/s13530-016-0256-2

Cite this

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title = "Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin",

abstract = "A high quantity of melanin causes hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes much melanin. In this study, lysosomal proteins were identified through two-dimensional polyacrylamide gel electrophoresis (2-DE) after melanocytes were exposed to melanin (B16F10). Melanocytes were exposed to 100 ppm melanin for 24 hours and the lysosomal proteins were extracted. The results showed that 18 protein spots up regulated and 6 protein spots down regulated after melanin exposure. The expression pattern of established lysosomal proteins is informative in understanding proteins related to decreasing melanin.",

keywords = "B16F10, Lysosomal proteins, Melanin, Melanocyte, Two-dimensional electrophoresis (2-DE)",

author = "Park, \{Dong Jun\} and Sekhon, \{Simranjeet Singh\} and Ahn, \{Ji Young\} and Hobaek Yoon and Ko, \{Jung Ho\} and Lyon Lee and Kim, \{Yang Hoon\} and Jiho Min",

note = "Publisher Copyright: {\textcopyright} 2016, Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.",

year = "2016",

month = mar,

day = "1",

doi = "10.1007/s13530-016-0256-2",

language = "English",

volume = "8",

pages = "7--11",

journal = "Toxicology and Environmental Health Sciences",

issn = "2005-9752",

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TY - JOUR

T1 - Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin

AU - Park, Dong Jun

AU - Sekhon, Simranjeet Singh

AU - Ahn, Ji Young

AU - Yoon, Hobaek

AU - Ko, Jung Ho

AU - Lee, Lyon

AU - Kim, Yang Hoon

AU - Min, Jiho

PY - 2016/3/1

Y1 - 2016/3/1

N2 - A high quantity of melanin causes hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes much melanin. In this study, lysosomal proteins were identified through two-dimensional polyacrylamide gel electrophoresis (2-DE) after melanocytes were exposed to melanin (B16F10). Melanocytes were exposed to 100 ppm melanin for 24 hours and the lysosomal proteins were extracted. The results showed that 18 protein spots up regulated and 6 protein spots down regulated after melanin exposure. The expression pattern of established lysosomal proteins is informative in understanding proteins related to decreasing melanin.

AB - A high quantity of melanin causes hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes much melanin. In this study, lysosomal proteins were identified through two-dimensional polyacrylamide gel electrophoresis (2-DE) after melanocytes were exposed to melanin (B16F10). Melanocytes were exposed to 100 ppm melanin for 24 hours and the lysosomal proteins were extracted. The results showed that 18 protein spots up regulated and 6 protein spots down regulated after melanin exposure. The expression pattern of established lysosomal proteins is informative in understanding proteins related to decreasing melanin.

KW - B16F10

KW - Lysosomal proteins

KW - Melanin

KW - Melanocyte

KW - Two-dimensional electrophoresis (2-DE)

UR - https://www.scopus.com/pages/publications/84969916978

U2 - 10.1007/s13530-016-0256-2

DO - 10.1007/s13530-016-0256-2

M3 - Journal article

AN - SCOPUS:84969916978

SN - 2005-9752

VL - 8

SP - 7

EP - 11

JO - Toxicology and Environmental Health Sciences

JF - Toxicology and Environmental Health Sciences

IS - 1

ER -

Proteomic analysis of lysosomal proteins in melanocyte B16F10 exposed to melanin

Abstract

UN SDGs

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

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