Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis

Yong Hwan Han; Sung Zoo Kim; Suhn Hee Kim; Woo Hyun Park

doi:10.1016/j.cbi.2008.10.014

Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis

Yong Hwan Han
, Sung Zoo Kim
, Suhn Hee Kim
, Woo Hyun Park^*

^*Corresponding author for this work

Department of Medicine

Centers for Healthcare Technology Development

Research output: Contribution to journal › Journal article › peer-review

54 Scopus citations

Abstract

Pyrogallol (PG) is a polyphenol compound and a known O₂{radical dot}^- generator. We evaluated the effects of PG on the growth and apoptosis of human pulmonary adenocarcinoma Calu-6 cells. PG decreased the viability of Calu-6 cells in a dose- and time-dependent manner. The induction of apoptosis by PG was accompanied by the loss of mitochondrial membrane potential (ΔΨ_m), cytochrome c release from mitochondria and activation of caspase-3 and caspase-8. All tested caspase inhibitors, especially the pan-caspase inhibitor (Z-VAD), markedly rescued Calu-6 cells from PG-induced cell death. Rescue was accompanied by inhibition of caspase-3 activation and PARP cleavage. Treatment with Z-VAD also prevented the loss of mitochondrial membrane potential (ΔΨ_m). In conclusion, PG inhibits the growth of Calu-6 cells via caspase-dependent apoptosis.

Original language	English
Pages (from-to)	107-114
Number of pages	8
Journal	Chemico-Biological Interactions
Volume	177
Issue number	2
DOIs	https://doi.org/10.1016/j.cbi.2008.10.014
State	Published - 2009.01.27

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Calu-6
Caspase
Pyrogallol
ROS

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10.1016/j.cbi.2008.10.014

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title = "Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis",

abstract = "Pyrogallol (PG) is a polyphenol compound and a known O2\{radical dot\}- generator. We evaluated the effects of PG on the growth and apoptosis of human pulmonary adenocarcinoma Calu-6 cells. PG decreased the viability of Calu-6 cells in a dose- and time-dependent manner. The induction of apoptosis by PG was accompanied by the loss of mitochondrial membrane potential (ΔΨm), cytochrome c release from mitochondria and activation of caspase-3 and caspase-8. All tested caspase inhibitors, especially the pan-caspase inhibitor (Z-VAD), markedly rescued Calu-6 cells from PG-induced cell death. Rescue was accompanied by inhibition of caspase-3 activation and PARP cleavage. Treatment with Z-VAD also prevented the loss of mitochondrial membrane potential (ΔΨm). In conclusion, PG inhibits the growth of Calu-6 cells via caspase-dependent apoptosis.",

keywords = "Apoptosis, Calu-6, Caspase, Pyrogallol, ROS",

author = "Han, \{Yong Hwan\} and Kim, \{Sung Zoo\} and Kim, \{Suhn Hee\} and Park, \{Woo Hyun\}",

year = "2009",

month = jan,

day = "27",

doi = "10.1016/j.cbi.2008.10.014",

language = "English",

volume = "177",

pages = "107--114",

journal = "Chemico-Biological Interactions",

issn = "0009-2797",

number = "2",

}

TY - JOUR

T1 - Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis

AU - Han, Yong Hwan

AU - Kim, Sung Zoo

AU - Kim, Suhn Hee

AU - Park, Woo Hyun

PY - 2009/1/27

Y1 - 2009/1/27

N2 - Pyrogallol (PG) is a polyphenol compound and a known O2{radical dot}- generator. We evaluated the effects of PG on the growth and apoptosis of human pulmonary adenocarcinoma Calu-6 cells. PG decreased the viability of Calu-6 cells in a dose- and time-dependent manner. The induction of apoptosis by PG was accompanied by the loss of mitochondrial membrane potential (ΔΨm), cytochrome c release from mitochondria and activation of caspase-3 and caspase-8. All tested caspase inhibitors, especially the pan-caspase inhibitor (Z-VAD), markedly rescued Calu-6 cells from PG-induced cell death. Rescue was accompanied by inhibition of caspase-3 activation and PARP cleavage. Treatment with Z-VAD also prevented the loss of mitochondrial membrane potential (ΔΨm). In conclusion, PG inhibits the growth of Calu-6 cells via caspase-dependent apoptosis.

AB - Pyrogallol (PG) is a polyphenol compound and a known O2{radical dot}- generator. We evaluated the effects of PG on the growth and apoptosis of human pulmonary adenocarcinoma Calu-6 cells. PG decreased the viability of Calu-6 cells in a dose- and time-dependent manner. The induction of apoptosis by PG was accompanied by the loss of mitochondrial membrane potential (ΔΨm), cytochrome c release from mitochondria and activation of caspase-3 and caspase-8. All tested caspase inhibitors, especially the pan-caspase inhibitor (Z-VAD), markedly rescued Calu-6 cells from PG-induced cell death. Rescue was accompanied by inhibition of caspase-3 activation and PARP cleavage. Treatment with Z-VAD also prevented the loss of mitochondrial membrane potential (ΔΨm). In conclusion, PG inhibits the growth of Calu-6 cells via caspase-dependent apoptosis.

KW - Apoptosis

KW - Calu-6

KW - Caspase

KW - Pyrogallol

KW - ROS

UR - https://www.scopus.com/pages/publications/57749202693

U2 - 10.1016/j.cbi.2008.10.014

DO - 10.1016/j.cbi.2008.10.014

M3 - Journal article

C2 - 19000662

AN - SCOPUS:57749202693

SN - 0009-2797

VL - 177

SP - 107

EP - 114

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

IS - 2

ER -

Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis

Abstract

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Keywords

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