Reclassification of Aeromonas sharmana to a new genus as Pseudaeromonas sharmana gen. Nov., comb. nov., and description of Pseudaeromonas pectinilytica sp. nov. isolated from a freshwater stream

A Gram-reaction-negative, rod-shaped, facultatively anaerobic, motile bacterium, designated strain AR1^T, was isolated from a freshwater stream in Jeonju, South Korea. Strain AR1^T showed highest 16S rRNA gene sequence similarity (96.83 %) and also formed a separate clade with Aeromonas sharmana GPTSA-6^T in the phylogenetic tree reconstructed among the members of the family Aeromonadaceae. Major cellular fatty acids are summed feature 3 (C_{16: 1}ω7c/C_{16: 1}ω6c) and C_{16: 0}. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol are the predominant polar lipids. The genomic DNA G+C content was found to be 54.7 mol%. However, earlier studies on 16S rRNA gene, gyrB, rpoD and universal target region of cpn60 sequences of the members of the genus Aeromonas recommended the transfer of Aeromonas sharmana to a new genus. Hence, based on the comparative polyphasic data obtained during the present study and also on the previous recommendations, it is proposed that Aeromonas sharmana be transferred to a novel genus as Pseudaeromonas sharmana gen. nov., comb. nov. with strain GPTSA-6^T (=DSM 17445^T=MTCC 7090^T=CIP 109378^T=CCUG 54939^T) as the type strain of the type species of the genus. Also, it is proposed that strain AR1T be designated as a representative of a novel species of this new genus, namely Pseudaeromonas pectinilytica sp. nov. The type strain is AR1T (=KCTC 42754^T=JCM 31503^T).