Regulation of proliferation of mouse bone marrow-derived mast cells by activated fibroblasts

Sung Joo Park; Hyung Ryong Kim; Hye Won Cho; Hyung Min Kim

doi:10.1007/bf02976380

Regulation of proliferation of mouse bone marrow-derived mast cells by activated fibroblasts

Sung Joo Park
, Hyung Ryong Kim
, Hye Won Cho
, Hyung Min Kim^*

^*Corresponding author for this work

Department of Dentistry

Wonkwang University

Research output: Contribution to journal › Journal article › peer-review

Abstract

Nitric oxide (NO) is synthesized by various cells involved in inflammatory reactions and may then act on mast cells. In the present work, we attempted to clarify the role of this molecule on the proliferation of mouse bone marrow derived-mast cells (BMMC). Swiss 3T3 fibroblasts produced nitrite (NO₂^-) and nitrate (NO₃^-) upon treatment with interferon γ (IFN-γ). This formation was dependent of L-arginine and could be inhibited by the L-arginine analogue N^G-monomethyl-L-arginine (N^GMMA). The effect of IFN-γ was drastically increased by cotreatment with tumor necrosis factor γ (TNF-γ). BMMC were maintained in vitro for as long as 30 days when cocultured with Swiss 3T3 fibroblasts. Coculture with N^GMMA, significantly increased the number of BMMC. These results indicate that NO involves the inhibition of proliferation of BMMC when cocultured with Swiss 3T3 fibroblasts.

Original language	English
Pages (from-to)	368-373
Number of pages	6
Journal	Archives of Pharmacal Research
Volume	19
Issue number	5
DOIs	https://doi.org/10.1007/bf02976380
State	Published - 1996.10

Keywords

Interferon-γ
Mouse bone marrow derived-mast cells
Nitric oxide
Swiss 3T3 fibroblasts
Tumor necrosis factor-α

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10.1007/bf02976380

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title = "Regulation of proliferation of mouse bone marrow-derived mast cells by activated fibroblasts",

abstract = "Nitric oxide (NO) is synthesized by various cells involved in inflammatory reactions and may then act on mast cells. In the present work, we attempted to clarify the role of this molecule on the proliferation of mouse bone marrow derived-mast cells (BMMC). Swiss 3T3 fibroblasts produced nitrite (NO2-) and nitrate (NO3-) upon treatment with interferon γ (IFN-γ). This formation was dependent of L-arginine and could be inhibited by the L-arginine analogue NG-monomethyl-L-arginine (NGMMA). The effect of IFN-γ was drastically increased by cotreatment with tumor necrosis factor γ (TNF-γ). BMMC were maintained in vitro for as long as 30 days when cocultured with Swiss 3T3 fibroblasts. Coculture with NGMMA, significantly increased the number of BMMC. These results indicate that NO involves the inhibition of proliferation of BMMC when cocultured with Swiss 3T3 fibroblasts.",

keywords = "Interferon-γ, Mouse bone marrow derived-mast cells, Nitric oxide, Swiss 3T3 fibroblasts, Tumor necrosis factor-α",

author = "Park, \{Sung Joo\} and Kim, \{Hyung Ryong\} and Cho, \{Hye Won\} and Kim, \{Hyung Min\}",

year = "1996",

month = oct,

doi = "10.1007/bf02976380",

language = "English",

volume = "19",

pages = "368--373",

journal = "Archives of Pharmacal Research",

issn = "0253-6269",

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}

TY - JOUR

T1 - Regulation of proliferation of mouse bone marrow-derived mast cells by activated fibroblasts

AU - Park, Sung Joo

AU - Kim, Hyung Ryong

AU - Cho, Hye Won

AU - Kim, Hyung Min

PY - 1996/10

Y1 - 1996/10

N2 - Nitric oxide (NO) is synthesized by various cells involved in inflammatory reactions and may then act on mast cells. In the present work, we attempted to clarify the role of this molecule on the proliferation of mouse bone marrow derived-mast cells (BMMC). Swiss 3T3 fibroblasts produced nitrite (NO2-) and nitrate (NO3-) upon treatment with interferon γ (IFN-γ). This formation was dependent of L-arginine and could be inhibited by the L-arginine analogue NG-monomethyl-L-arginine (NGMMA). The effect of IFN-γ was drastically increased by cotreatment with tumor necrosis factor γ (TNF-γ). BMMC were maintained in vitro for as long as 30 days when cocultured with Swiss 3T3 fibroblasts. Coculture with NGMMA, significantly increased the number of BMMC. These results indicate that NO involves the inhibition of proliferation of BMMC when cocultured with Swiss 3T3 fibroblasts.

AB - Nitric oxide (NO) is synthesized by various cells involved in inflammatory reactions and may then act on mast cells. In the present work, we attempted to clarify the role of this molecule on the proliferation of mouse bone marrow derived-mast cells (BMMC). Swiss 3T3 fibroblasts produced nitrite (NO2-) and nitrate (NO3-) upon treatment with interferon γ (IFN-γ). This formation was dependent of L-arginine and could be inhibited by the L-arginine analogue NG-monomethyl-L-arginine (NGMMA). The effect of IFN-γ was drastically increased by cotreatment with tumor necrosis factor γ (TNF-γ). BMMC were maintained in vitro for as long as 30 days when cocultured with Swiss 3T3 fibroblasts. Coculture with NGMMA, significantly increased the number of BMMC. These results indicate that NO involves the inhibition of proliferation of BMMC when cocultured with Swiss 3T3 fibroblasts.

KW - Interferon-γ

KW - Mouse bone marrow derived-mast cells

KW - Nitric oxide

KW - Swiss 3T3 fibroblasts

KW - Tumor necrosis factor-α

UR - https://www.scopus.com/pages/publications/20544454600

U2 - 10.1007/bf02976380

DO - 10.1007/bf02976380

M3 - Journal article

AN - SCOPUS:20544454600

SN - 0253-6269

VL - 19

SP - 368

EP - 373

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 5

ER -

Regulation of proliferation of mouse bone marrow-derived mast cells by activated fibroblasts

Abstract

Keywords

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