Regulation of [3H]norepinephrine release by opioids in human cerebral cortex

Ran Sook Woo; Byoung Soo Shin; Chul Jin Kim; Min Soo Shin; Min Suk Jeong; Rong Jie Zhao; Kee Won Kim

Regulation of [³H]norepinephrine release by opioids in human cerebral cortex

Ran Sook Woo
, Byoung Soo Shin
, Chul Jin Kim
, Min Soo Shin
, Min Suk Jeong
, Rong Jie Zhao
, Kee Won Kim^*

^*Corresponding author for this work

Department of Medicine

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

Abstract

To investigate the receptors mediating the regulation of norepinephrine (NE) release in human cerebral cortex slices, we examined the effects of opioid agonists for μ-, δ-, and κ-receptors on the high potassium (15 mM)-evoked release of [³H]NE. [³H]NE release induced by high potassium was calcium-dependent and tetrodotoxin-sensitive. [D-Pen², D-Pen⁵]enkephalin (DPDPE) and deltorphin II (Delt II) inhibited the stimulated release of norepinephrine in a dose-dependent manner. However, Tyr-D-Ala-Gly-(Me)Phe-Gly-ol and U69,593 did not influence the NE release. Inhibitory effect of DPDPE and Delt-II was antagonized by naloxone, naltrindole, 7-benzylidenaltrexone and naltriben. These results suggest that both δ₁ and δ₂ receptors are involved in regulation of NE release in human cerebral cortex.

Original language	English
Pages (from-to)	1-3
Number of pages	3
Journal	Korean Journal of Physiology and Pharmacology
Volume	7
Issue number	1
State	Published - 2003.02

Keywords

Human cerebral cortex
Norepinephrine release
Opioid receptors

Quacquarelli Symonds(QS) Subject Topics

Anatomy & Physiology
Pharmacy & Pharmacology

Cite this

@article{309f55309d544b90977cb5610ed25b13,

title = "Regulation of [3H]norepinephrine release by opioids in human cerebral cortex",

abstract = "To investigate the receptors mediating the regulation of norepinephrine (NE) release in human cerebral cortex slices, we examined the effects of opioid agonists for μ-, δ-, and κ-receptors on the high potassium (15 mM)-evoked release of [3H]NE. [3H]NE release induced by high potassium was calcium-dependent and tetrodotoxin-sensitive. [D-Pen2, D-Pen5]enkephalin (DPDPE) and deltorphin II (Delt II) inhibited the stimulated release of norepinephrine in a dose-dependent manner. However, Tyr-D-Ala-Gly-(Me)Phe-Gly-ol and U69,593 did not influence the NE release. Inhibitory effect of DPDPE and Delt-II was antagonized by naloxone, naltrindole, 7-benzylidenaltrexone and naltriben. These results suggest that both δ1 and δ2 receptors are involved in regulation of NE release in human cerebral cortex.",

keywords = "Human cerebral cortex, Norepinephrine release, Opioid receptors",

author = "Woo, \{Ran Sook\} and Shin, \{Byoung Soo\} and Kim, \{Chul Jin\} and Shin, \{Min Soo\} and Jeong, \{Min Suk\} and Zhao, \{Rong Jie\} and Kim, \{Kee Won\}",

year = "2003",

month = feb,

language = "English",

volume = "7",

pages = "1--3",

journal = "Korean Journal of Physiology and Pharmacology",

issn = "1226-4512",

number = "1",

}

TY - JOUR

T1 - Regulation of [3H]norepinephrine release by opioids in human cerebral cortex

AU - Woo, Ran Sook

AU - Shin, Byoung Soo

AU - Kim, Chul Jin

AU - Shin, Min Soo

AU - Jeong, Min Suk

AU - Zhao, Rong Jie

AU - Kim, Kee Won

PY - 2003/2

Y1 - 2003/2

N2 - To investigate the receptors mediating the regulation of norepinephrine (NE) release in human cerebral cortex slices, we examined the effects of opioid agonists for μ-, δ-, and κ-receptors on the high potassium (15 mM)-evoked release of [3H]NE. [3H]NE release induced by high potassium was calcium-dependent and tetrodotoxin-sensitive. [D-Pen2, D-Pen5]enkephalin (DPDPE) and deltorphin II (Delt II) inhibited the stimulated release of norepinephrine in a dose-dependent manner. However, Tyr-D-Ala-Gly-(Me)Phe-Gly-ol and U69,593 did not influence the NE release. Inhibitory effect of DPDPE and Delt-II was antagonized by naloxone, naltrindole, 7-benzylidenaltrexone and naltriben. These results suggest that both δ1 and δ2 receptors are involved in regulation of NE release in human cerebral cortex.

AB - To investigate the receptors mediating the regulation of norepinephrine (NE) release in human cerebral cortex slices, we examined the effects of opioid agonists for μ-, δ-, and κ-receptors on the high potassium (15 mM)-evoked release of [3H]NE. [3H]NE release induced by high potassium was calcium-dependent and tetrodotoxin-sensitive. [D-Pen2, D-Pen5]enkephalin (DPDPE) and deltorphin II (Delt II) inhibited the stimulated release of norepinephrine in a dose-dependent manner. However, Tyr-D-Ala-Gly-(Me)Phe-Gly-ol and U69,593 did not influence the NE release. Inhibitory effect of DPDPE and Delt-II was antagonized by naloxone, naltrindole, 7-benzylidenaltrexone and naltriben. These results suggest that both δ1 and δ2 receptors are involved in regulation of NE release in human cerebral cortex.

KW - Human cerebral cortex

KW - Norepinephrine release

KW - Opioid receptors

UR - https://www.scopus.com/pages/publications/0037318174

M3 - Journal article

AN - SCOPUS:0037318174

SN - 1226-4512

VL - 7

SP - 1

EP - 3

JO - Korean Journal of Physiology and Pharmacology

JF - Korean Journal of Physiology and Pharmacology

IS - 1

ER -

Regulation of [³H]norepinephrine release by opioids in human cerebral cortex

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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