Reinforcement of carboxyl groups in the surface of Corynebacterium glutamicum biomass for effective removal of basic dyes

The biomass of Corynebacterium glutamicum was treated with poly(amic acid) to improve the biosorption of Basic Blue 3 (BB3) from aqueous solution. The grafting of poly(amic acid) onto the biomass surface increased the density of the carboxyl groups. The UV-spectrum revealed that strong acidic (pH ≤ 2) and basic conditions (pH ≥ 11) resulted in the precipitation of BB3. Therefore, pH edge experiments were conducted only within the range 3-10; these results indicated that electrostatic attraction between carboxyl groups of C. glutamicum and BB3 dye cations was favored under alkaline conditions. From the Langmuir model, poly(amic acid)-modified biomass gave a maximum uptake of 173.6 mg/g at pH 9, compared to 52.8 mg/g by the raw biomass. The biosorption kinetics was found to be fast; with equilibrium attained within 10 min. The increase in the ionic strength strongly affected the uptake of BB3 for both forms of C. glutamicum.